Simultaneous quantitation of sphingoid bases by UPLC-ESI-MS/MS with identical13C-encoded internal standards
| Autor: | Maria J. Ferraz, Jeroen D. C. Codée, Kassiani Kytidou, André R. A. Marques, P. Gaspar, Patrick Wisse, Mina Mirzaian, G. A. Van Der Marel, Johannes M. F. G. Aerts, Saskia V. Oussoren, Herman S. Overkleeft |
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| Přispěvatelé: | ACS - Amsterdam Cardiovascular Sciences, Medical Biochemistry, AGEM - Amsterdam Gastroenterology Endocrinology Metabolism |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Clinical Biochemistry Biochemistry Mass Spectrometry Sphingolipidoses 03 medical and health sciences chemistry.chemical_compound Lactosylsphingosine 0302 clinical medicine Tandem Mass Spectrometry Quantification medicine Humans Multiplex Chromatography High Pressure Liquid Carbon Isotopes Sphingolipids Chromatography Gaucher Disease Plasma samples Sphingosine Biochemistry (medical) Uplc esi ms ms General Medicine Sphingoid Bases Reference Standards medicine.disease Sphingolipid C-13-Encoded Internal Standards Doenças Genéticas 030104 developmental biology chemistry Lysosphingolipids Fabry Disease Female 030217 neurology & neurosurgery |
| Zdroj: | Clinica chimica acta; international journal of clinical chemistry, 466, 178-184. Elsevier Clinica Chimica Acta, 466, 178-184 |
| ISSN: | 0009-8981 |
| Popis: | Free sphingoid bases (lysosphingolipids) of primary storage sphingolipids are increased in tissues and plasma of several sphingolipidoses. As shown earlier by us, sphingoid bases can be accurately quantified using UPLC-ESI-MS/MS, particularly in combination with identical13C-encoded internal standards. The feasibility of simultaneous quantitation of sphingoid bases in plasma specimens spiked with a mixture of such standards is here described. The sensitivity and linearity of detection is excellent for all examined sphingoid bases (sphingosine, sphinganine, hexosyl-sphingosine (glucosylsphingosine), hexosyl2-sphingosine (lactosylsphingosine), hexosyl3-sphingosine (globotriaosylsphingosine), phosphorylcholine-sphingosine) in the relevant concentration range and the measurements show very acceptable intra- and inter-assay variation ( |
| Databáze: | OpenAIRE |
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