Dual-modality optical coherence tomography and frequency-domain fluorescence lifetime imaging microscope system for intravascular imaging
| Autor: | Michael J. Serafino, Brian E. Applegate, Zheng Tan, Wihan Kim, Xi Chen, Javier A. Jo |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Paper
Fluorescence-lifetime imaging microscopy Microscope Materials science Endoscope Biomedical Engineering Imaging law.invention Biomaterials Optical coherence tomography law medicine Humans endoscopy fiber optic applications Image resolution optical coherence tomography medicine.diagnostic_test Optical Imaging fluorescence spectroscopy Cladding (fiber optics) Coronary Vessels Plaque Atherosclerotic Atomic and Molecular Physics and Optics Electronic Optical and Magnetic Materials Endoscopy Coronary arteries medicine.anatomical_structure Microscopy Fluorescence atherosclerosis Tomography Optical Coherence Biomedical engineering |
| Zdroj: | Journal of Biomedical Optics |
| ISSN: | 1083-3668 |
| DOI: | 10.1117/1.jbo.25.9.096010 |
| Popis: | Significance: Detailed biochemical and morphological imaging of the plaque burdened coronary arteries holds the promise of improved understanding of atherosclerosis plaque development, ultimately leading to better diagnostics and therapies. Aim: Development of a dual-modality intravascular catheter supporting swept-source optical coherence tomography (OCT) and frequency-domain fluorescence lifetime imaging (FD-FLIM) of endogenous fluorophores with UV excitation. Approach: We instituted a refined approach to endoscope development that combines simulation in a commercial ray tracing program, fabrication, and a measurement method for optimizing ball-lens performance. With this approach, we designed and developed a dual-modality catheter endoscope based on a double-clad fiber supporting OCT through the core and fluorescence collection through the first cladding. We varied the relative percent of UV excitation launched into the core and first cladding to explore the potential resolution improvement for FD-FLIM. The developed catheter endoscope was optically characterized, including measurement of spatial resolution and fluorescent lifetimes of standard fluorophores. Finally, the system was demonstrated on fresh ex vivo human coronary arteries. Results: The developed endoscope was shown to have optical performance similar to predictions derived from the simulation approach. The FLIM resolution can be improved by over a factor of 4 by primarily illuminating through the core rather than the first cladding. However, time-dependent solarization losses need to be considered when choosing the relative percentage. We ultimately chose to illuminate with 7% of the power transmitting through the core. The resulting catheter endoscope had 40-μm lateral resolution for OCT and |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|