Effect of Pin1 inhibitor juglone on proliferation, migration and angiogenic ability of breast cancer cell line MCF7Adr
| Autor: | Yuan-gui Hu, Yun-feng Shen, Yi Li |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Vascular Endothelial Growth Factor A
Cyclin E Angiogenesis Biomedical Engineering Antineoplastic Agents Breast Neoplasms Biochemistry Biomaterials Andrology chemistry.chemical_compound Cell Movement Genetics Humans Medicine Cell Proliferation Earth-Surface Processes Peptidylprolyl isomerase business.industry Cell growth Cell Cycle Peptidylprolyl Isomerase Cell cycle Gene Expression Regulation Neoplastic NIMA-Interacting Peptidylprolyl Isomerase Vascular endothelial growth factor Vascular endothelial growth factor A chemistry Immunology MCF-7 Cells Female business Juglone Naphthoquinones |
| Zdroj: | Journal of Huazhong University of Science and Technology [Medical Sciences]. 35:531-534 |
| ISSN: | 1993-1352 1672-0733 |
| DOI: | 10.1007/s11596-015-1465-7 |
| Popis: | This study aimed to evaluate the effects of Pin1 inhibitor Juglone on proliferation, migration and the angiogenic ability of breast cancer cell line MCF7Adr. MCF7Adr cells were cultured and separately treated with Pin1 inhibitor Juglone (treatment group) and DMEM without drug (control group). The cell cycle was examined by flow cytometry. Cell migration was measured by wound-healing assay. Cyclin E protein content was detected by Western blotting. The angiogenesis factor vascular endothelial growth factor (VEGF) in cell media was determined by enzyme linked immunosorbent assay. The results showed that the percentage of cells in G2/M phase in treatment group was significantly higher than that in control group (25.5% vs. 10.1%, P |
| Databáze: | OpenAIRE |
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