Effects of Different Concentrations of Injectable Collagenase Enzyme on Capsular Tissue Around Silicone Implants: A Preliminary Experimental Study for the Development of a New Treatment Strategy

Autor: Hakan Şirinoğlu, Murat Sarici, Kemalettin Yildiz, Gökhan Temiz, Nebil Yeşiloğlu, Gaye Taylan Filinte, Ali Cem Akpinar, Emre Güvercin, Deniz Filinte
Přispěvatelé: YILDIZ, KEMALETTİN
Rok vydání: 2015
Předmět:
medicine.medical_specialty
Breast Implants
030230 surgery
Injections
Intralesional
Rats
Sprague-Dawley
Silicone Gels
03 medical and health sciences
chemistry.chemical_compound
Random Allocation
0302 clinical medicine
Silicone
Clostridium histolyticum
Implant Capsular Contracture
medicine
Animals
Collagenases
Rats
Wistar
A Preliminary Experimental Study for the Development of a New Treatment Strategy-
AESTHETIC PLASTIC SURGERY
cilt.40
ss.164-173
2016 [Yesiloglu N.
Temiz G.
Sarici M.
YILDIZ K.
Sirinoglu H.
Guvercin E.
Akpinar A. C.
Filinte G. T.
FİLİNTE D.
-Effects of Different Concentrations of Injectable Collagenase Enzyme on Capsular Tissue Around Silicone Implants]
biology
business.industry
Capsule
Capsular contracture
biology.organism_classification
medicine.disease
Surgery
Rats
Plastic surgery
chemistry
030220 oncology & carcinogenesis
Seroma
Collagenase
Female
business
medicine.drug
Zdroj: Aesthetic plastic surgery. 40(1)
ISSN: 1432-5241
Popis: In recent studies, collagen organization was blamed for the formation of capsular contracture which is still a challenging problem after silicone implant-based breast operations. In this study, effects of different concentrations of collagenase enzyme derived from Clostridium histolyticum on the capsular tissue formation around the silicone implants were investigated. The injectable form of collagenase has a routine clinical use in the treatment of both Dupuytren’s and Peyronie’s diseases. Thirty-two Wistar albino rats were randomized into four groups. A 2 × 1 × 0.3-cm-sized silicone block was inserted inside a dorsal subcutaneous pocket in all groups. After 2 months of insertion, capsule thicknesses around the implants were detected under ultrasonography. This was followed by injection of isotonic saline, 150, 300, and 600 IU in Gr-1, 2, 3, and 4, respectively. All the animals were sacrificed at the end of the first week for histologic sampling to determine fibroblast proliferation, vessel density of the tissue, necrosis, edema, inflammation, and capsule thickness. All the data were statistically analyzed using Kruskal–Wallis and Mann–Whitney U tests and compared for significance of the results. There was no significant difference in terms of capsule thinning between the 300 and 600 IU groups but in both groups thinning was significantly higher than the sham group. In the 150 IU group there was no significant thinning as compared to the sham group (p > 0.05). However, complications such as skin necrosis, infection, and seroma formation were seen only in the 600 IU injection group. The optimal safe and effective dose of the enzyme was accepted as 300 IU. The 300 IU injection provided up to 89 % thinning in the capsule tissue. There was thinning of the collagen bundles parallel to capsule thickness. In the 600 IU group, micro-pores were encountered at the thinnest points. However, the late results and recurrence rates of capsular contracture were not included in this study; collagenase seemed effective for the reduction of capsular tissue around the implants. This journal requires that authors assign a level of evidence to each submission to which Evidence-Based Medicine rankings are applicable. This excludes Review Articles, Book Reviews, and manuscripts that concern Basic Science, Animal Studies, Cadaver Studies, and Experimental Studies. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .
Databáze: OpenAIRE
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