Electrochemical quartz crystal microbalance with dissipation investigation of fibronectin adsorption dynamics driven by electrical stimulation onto a conducting and partially biodegradable copolymer
| Autor: | Luiz Henrique Catalani, Aruã C. da Silva, Paula Montoya, Ricardo Bentini, Tatiana Augusto, Rubens A. Silva, Maria J P G Souza, Susana I. Córdoba de Torresi, Roberto M. Torresi |
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| Rok vydání: | 2020 |
| Předmět: |
Materials science
Polymers General Physics and Astronomy Biocompatible Materials 02 engineering and technology 010402 general chemistry Electrochemistry 01 natural sciences General Biochemistry Genetics and Molecular Biology Biomaterials Mice Adsorption Tissue engineering Elastic Modulus Copolymer Animals General Materials Science Electrical conductor chemistry.chemical_classification FIBRONECTINAS General Chemistry Quartz crystal microbalance Polymer Fibroblasts 021001 nanoscience & nanotechnology Electric Stimulation 0104 chemical sciences Fibronectins chemistry Chemical engineering Drug delivery NIH 3T3 Cells Quartz Crystal Microbalance Techniques 0210 nano-technology |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1559-4106 |
| Popis: | Functional surface coatings are a key option for biomedical applications, from polymeric supports for tissue engineering to smart matrices for controlled drug delivery. Therefore, the synthesis of new materials for biological applications and developments is promising. Hence, biocompatible and stimuli-responsive polymers are interesting materials, especially when they present conductive properties. PEDOT-co-PDLLA graft copolymer exhibits physicochemical and mechanical characteristics required for biomedical purposes, associated with electroactive, biocompatible, and partially biodegradable properties. Herein, the study of fibronectin (FN) adsorption onto PEDOT-co-PDLLA carried out by an electrochemical quartz crystal microbalance with dissipation is reported. The amount of FN adsorbed onto PEDOT-co-PDLLA was higher than that adsorbed onto the Au surface, with a significant increase when electrical stimulation was applied (either at +0.5 or −0.125 V). Additionally, FN binds to the copolymer interface in an unfolded conformation, which can promote better NIH-3T3 fibroblast cell adhesion and later cell development. |
| Databáze: | OpenAIRE |
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