Paper-based magnetic nanoparticle-peptide probe for rapid and quantitative colorimetric detection of Escherichia coli O157:H7
| Autor: | Ghadeer A. R. Y. Suaifan, Sahar Alhogail, Mohammed Zourob |
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| Rok vydání: | 2017 |
| Předmět: |
Meat
Turkey Biomedical Engineering Biophysics Nanoparticle Food Contamination Peptide Nanotechnology Biosensing Techniques 02 engineering and technology Escherichia coli O157 medicine.disease_cause 01 natural sciences Foodborne Diseases Limit of Detection Electrochemistry medicine Animals Humans Magnetite Nanoparticles Escherichia coli Escherichia coli Infections Detection limit chemistry.chemical_classification Chromatography 010401 analytical chemistry General Medicine Paper based Lettuce 021001 nanoscience & nanotechnology 0104 chemical sciences Milk chemistry Food Microbiology Cattle Colorimetry Naked eye Gradual increase Peptides 0210 nano-technology Biosensor Peptide Hydrolases Biotechnology |
| Zdroj: | Biosensors and Bioelectronics. 92:702-708 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/j.bios.2016.10.023 |
| Popis: | There is a critical and urgent demand for a simple, rapid and specific qualitative and quantitative colorimetric biosensor for the detection of the food contaminant Escherichia coli O157:H7 (E. coli O157:H7) in complex food products due to the recent outbreaks of food-borne diseases. Traditional detection techniques are time-consuming, require expensive instrumentation and are labour-intensive. To overcome these limitations, a novel, ultra-rapid visual biosensor was developed based on the ability of E. coli O157:H7 proteases to change the optical response of a surface-modified, magnetic nanoparticle-specific (MNP-specific) peptide probe. Upon proteolysis, a gradual increase in the golden color of the sensor surface was visually observed. The intensification of color was correlated with the E. coli O157:H7 concentration. The color change resulting from the dissociation of the self-assembled monolayer (SAM) was detected by the naked eye and analysed using an image analysis software (ImageJ) for the purpose of quantitative detection. This biosensor demonstrated high sensitivity and applicability, with lower limits of detection of 12 CFU mL−1 in broth samples and 30–300 CFU mL−1 in spiked complex food matrices. In conclusion, this approach permits the use of a disposable biosensor chip that can be mass-produced at low cost and can be used not only by food manufacturers but also by regulatory agencies for better control of potential health risks associated with the consumption of contaminated foods. |
| Databáze: | OpenAIRE |
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