In vitro-generated viral double-stranded RNA in contrast to polyinosinic:polycytidylic acid induces interferon-alpha in human plasmacytoid dendritic cells
| Autor: | Albrecht Bufe, Evelin Grage-Griebenow, Stefan Bauer, A. Wagner, Holger Heine, Shizuo Akira, S Löseke |
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| Rok vydání: | 2006 |
| Předmět: |
Transcription
Genetic viruses Immunology Plasma Cells Newcastle disease virus Alpha interferon Biology Transfection chemistry.chemical_compound Mice Interferon medicine Animals Humans RNA Double-Stranded Mice Knockout Innate immune system Dose-Response Relationship Drug Tumor Necrosis Factor-alpha fungi Interleukin-8 Toll-Like Receptors TLR9 RNA Interferon-alpha General Medicine TLR7 Dendritic Cells Molecular biology Poly I-C chemistry Toll-Like Receptor 7 Polyinosinic:polycytidylic acid RNA Viral medicine.drug |
| Zdroj: | Scandinavian journal of immunology. 63(4) |
| ISSN: | 0300-9475 |
| Popis: | Double-stranded RNA (dsRNA) arises in the cytoplasm during viral replication and was shown to participate in the interferon (IFN)-alpha induction process. Besides the intracellular recognition, released dsRNA from dying, infected cells can function as a pathogen-associated molecular pattern (PAMP) for the innate immune system. In the present study, in vitro-generated dsRNA fragments of genomic sequences of Newcastle disease virus were used to induce IFN-alpha release in human peripheral blood mononuclear cells (PBMC), in immature myeloid dendritic cells (mDC) and in immature plasmacytoid DC (pDC). The extracellular administration of dsRNA fragments but not the application of the corresponding single-stranded RNA (ssRNA) strands led to an IFN-alpha production in PBMC. The synthetic dsRNA analogue polyinosinic acid : polycytidylic acid [Poly(I : C)] could only stimulate IFN-alpha production in enriched mDC but not in pDC. In contrast, dsRNA fragments induced IFN-alpha only in pDC. Complexation of dsRNA fragments with transfection reagents increased the efficiency of IFN-alpha induction and commuted ssRNA molecules into IFN-alpha inducers. However, stimulation of in vitro-generated murine Toll-like receptor 7 (TLR7) knockout DC and human TLR-transfected HEK293 cells with dsRNA fragments gave no evidences for the involvement of pDC-specific TLR7 or TLR9 in the observed IFN-alpha induction. |
| Databáze: | OpenAIRE |
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