Timing of Transcriptional Quiescence during Gametogenesis Is Controlled by Global Histone H3K4 Demethylation
| Autor: | Mengshu Xu, Kyle Tsui, Corey Nislow, Marc D. Meneghini, Maria Soloveychik, Ryan M. Raisner, Timothy P. Hughes, Marinella Gebbia, Shivkumar Venkatasubrahmanyan, Zarna Shah, Mathieu Ranger, Hiten D. Madhani, Harm van Bakel, Michael R Schertzberg |
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| Jazyk: | angličtina |
| Předmět: |
Spores
Jumonji Domain-Containing Histone Demethylases Time Factors Transcription Genetic Cellular differentiation Messenger Medical and Health Sciences Gametogenesis Shelterin Complex Epigenesis Genetic Histones 0302 clinical medicine Transcription (biology) Genetics 0303 health sciences biology Biological Sciences Spores Fungal Nucleosomes Meiosis Fungal Histone Transcription Ribosomal Proteins Saccharomyces cerevisiae Proteins 1.1 Normal biological development and functioning Saccharomyces cerevisiae Genes Fungal Telomere-Binding Proteins Methylation General Biochemistry Genetics and Molecular Biology Article 03 medical and health sciences Genetic Underpinning research Nucleosome RNA Messenger Molecular Biology Transcription factor 030304 developmental biology Human Genome RNA Fungal Cell Biology biology.organism_classification Genes Mutation biology.protein RNA Demethylase 030217 neurology & neurosurgery Epigenesis Transcription Factors Developmental Biology |
| Zdroj: | Developmental cell, vol 23, iss 5 |
| ISSN: | 1534-5807 |
| DOI: | 10.1016/j.devcel.2012.10.005 |
| Popis: | SummaryGametes are among the most highly specialized cells produced during development. Although gametogenesis culminates in transcriptional quiescence in plants and animals, regulatory mechanisms controlling this are unknown. Here, we confirm that gamete differentiation in the single-celled yeast Saccharomyces cerevisiae is accompanied by global transcriptional shutoff following the completion of meiosis. We show that Jhd2, a highly conserved JARID1-family histone H3K4 demethylase, activates protein-coding gene transcription in opposition to this programmed transcriptional shutoff, sustaining the period of productive transcription during spore differentiation. Moreover, using genome-wide nucleosome, H3K4me, and transcript mapping experiments, we demonstrate that JHD2 globally represses intergenic noncoding transcription during this period. The widespread transcriptional defects of JHD2 mutants are associated with precocious differentiation and the production of stress-sensitive spores, demonstrating that Jhd2 regulation of the global postmeiotic transcriptional program is critical for the production of healthy meiotic progeny. |
| Databáze: | OpenAIRE |
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