Nucleotide Polymorphism in the Wheat Transcriptional Activator Spa Influences Its Pattern of Expression and Has Pleiotropic Effects on Grain Protein Composition, Dough Viscoelasticity, and Grain Hardness
| Autor: | Redouane El-Malki, Catherine Ravel, Pierre Martre, Isabelle Romeuf, Jacques Bordes, Mireille Dardevet, François Balfourier, Nathalie Duchateau, Dominique Brunel, Gilles Charmet |
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| Přispěvatelé: | Génétique Diversité et Ecophysiologie des Céréales (GDEC), Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP), Etude du Polymorphisme des Génomes Végétaux, Institut National de la Recherche Agronomique (INRA), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Institut National de la Recherche Agronomique (INRA) |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
0106 biological sciences
Time Factors Physiology Flour Plant Science 01 natural sciences Gliadin Linkage Disequilibrium Gene Expression Regulation Plant Gene expression Promoter Regions Genetic Triticum Plant Proteins 2. Zero hunger Genetics Regulation of gene expression 0303 health sciences Nucleotides Viscosity Gene Expression Regulation Developmental food and beverages Phenotype Seeds Research Article musculoskeletal diseases Nitrogen Molecular Sequence Data Sequence alignment Single-nucleotide polymorphism Flowers Biology [SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics 03 medical and health sciences Hardness Amino Acid Sequence Gene 030304 developmental biology Polymorphism Genetic Haplotype Promoter GENETIQUE Elasticity stomatognathic diseases Haplotypes Trans-Activators biology.protein Sequence Alignment 010606 plant biology & botany |
| Zdroj: | Plant Physiology Plant Physiology, Oxford University Press ; American Society of Plant Biologists, 2009, 151 (4), pp.2133-2144. ⟨10.1104/pp.109.146076⟩ Plant Physiology, American Society of Plant Biologists, 2009, 151 (4), pp.2133-2144. ⟨10.1104/pp.109.146076⟩ |
| ISSN: | 0032-0889 1532-2548 |
| DOI: | 10.1104/pp.109.146076⟩ |
| Popis: | Storage protein activator (SPA) is a key regulator of the transcription of wheat (Triticum aestivum) grain storage protein genes and belongs to the Opaque2 transcription factor subfamily. We analyzed the sequence polymorphism of the three homoeologous Spa genes in hexaploid wheat. The level of polymorphism in these genes was high particularly in the promoter. The deduced protein sequences of each homoeolog and haplotype show greater than 93% identity. Two major haplotypes were studied for each Spa gene. The three Spa homoeologs have similar patterns of expression during grain development, with a peak in expression around 300 degree days after anthesis. On average, Spa-B is 10 and seven times more strongly expressed than Spa-A and Spa-D, respectively. The haplotypes are associated with significant quantitative differences in Spa expression, especially for Spa-A and Spa-D. Significant differences were found in the quantity of total grain nitrogen allocated to the gliadin protein fractions for the Spa-A haplotypes, whereas the synthesis of glutenins is not modified. Genetic association analysis between Spa and dough viscoelasticity revealed that Spa polymorphisms are associated with dough tenacity, extensibility, and strength. Except for Spa-A, these associations can be explained by differences in grain hardness. No association was found between Spa markers and the average single grain dry mass or grain protein concentration. These results demonstrate that in planta Spa is involved in the regulation of grain storage protein synthesis. The associations between Spa and dough viscoelasticity and grain hardness strongly suggest that Spa has complex pleiotropic functions during grain development. |
| Databáze: | OpenAIRE |
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