Prevention of intestinal amebiasis by vaccination with the Entamoeba histolytica Gal/GalNac lectin
| Autor: | William A. Petri, David M. Lyerly, Lauren A. Lockhart, Rhonda L. Wright, Carole L. Cramer, Lisa Barroso, Eric R. Houpt |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Protozoan Vaccines
Cecal Infection Protozoan Proteins Antibodies Protozoan Antigens Protozoan CHO Cells Biology medicine.disease_cause Microbiology Feces Mice Entamoeba histolytica Antigen Cricetinae Lectins Cell Adhesion medicine Animals Cecum Administration Intranasal Mice Inbred BALB C Mice Inbred C3H General Veterinary General Immunology and Microbiology Vaccination Cholera toxin Public Health Environmental and Occupational Health Lectin Amebiasis biology.organism_classification Immunoglobulin A Intestines Infectious Diseases Immunization Immunology biology.protein Molecular Medicine Female Antibody Injections Intraperitoneal |
| Zdroj: | Vaccine. 22:611-617 |
| ISSN: | 0264-410X |
| DOI: | 10.1016/j.vaccine.2003.09.003 |
| Popis: | Prevention of intestinal infection by Entamoeba histolytica would block both invasive disease and parasite transmission. The amebic Gal/GalNAc lectin mediates parasite adherence to the colonic surface and fecal anti-lectin IgA is associated with protection from intestinal reinfection in children. We tested if vaccination with the E. histolytica Gal/GalNAc lectin could prevent cecal infection in a C3H mouse model of amebic colitis. Two trials using native lectin purified from the parasite and two trials using a 64 kDa recombinant fragment ("LecA") were performed with a combined intranasal and intraperitoneal immunization regimen using cholera toxin and Freund's adjuvants, respectively. Two weeks after immunization mice were challenged intracecally with trophozoites, and 4-12 weeks after challenge mice were sacrificed for histopathologic evaluation of infection. Vaccination prevented intestinal infection with efficacies of 84 and 100% in the two native lectin trials and 91 and 34% in the two LecA trials. Mice with detectable pre-challenge fecal anti-lectin IgA responses were significantly more resistant to infection than mice without fecal anti-lectin IgA responses. These results show for the first time that immunization with the Gal/GalNAc lectin can prevent intestinal amebiasis in mice and suggest a protective role for fecal anti-lectin IgA in vivo. |
| Databáze: | OpenAIRE |
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