The use of pan-tropomyosin receptor kinase immunohistochemistry as a screening tool for the detection of neurotrophic tropomyosin-related kinase fusions : real-world data from a national multicentric retrospective study
| Autor: | Mieke R. Van Bockstal, Gabriela Beniuga, Ligia Craciun, David Creytens, Franceska Dedeurwaerdere, Philippe Delvenne, Pieter Demetter, Bart De Wiest, Koen Dewinne, Lionel Habran, Patrick Pauwels, Ivan Theate, Sara Vander Borght, Kris Van Der Steen, Birgit Weynand |
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| Přispěvatelé: | UCL - SSS/IREC/SLUC - Pôle St.-Luc, UCL - (SLuc) Service d'anatomie pathologique |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
animal structures
CANCERS FREQUENCY Pathology and Forensic Medicine GENE FUSION Medicine and Health Sciences POSITIVE SOLID TUMORS Biology Molecular Biology NTRK FUSIONS REARRANGEMENTS Cell Biology General Medicine SALIVARY-GLAND Immunohistochemistry ENTRECTINIB enzymes and coenzymes (carbohydrates) TRK IMMUNOHISTOCHEMISTRY nervous system embryonic structures Neurotrophic tropomyosin-related kinase Next-generation sequencing Human medicine Pan-tropomyosin receptor kinase LAROTRECTINIB |
| Zdroj: | PATHOBIOLOGY Pathobiology, p. [1-14] (2022) Pathobiology |
| ISSN: | 1015-2008 1423-0291 |
| Popis: | Introduction: The neurotrophic tropomyosin-related kinase (NTRK) genes encode the tropomyosin receptor kinases (TRKs). Patients with solid tumors harboring an oncogenic NTRK fusion are eligible for treatment with TRK inhibitors. NTRK fusion is often associated with TRK overexpression. Pan-TRK immunohistochemistry (IHC) is used to screen for NTRK fusions, but immunoreactivity patterns are poorly defined. Methods: Data on pan-TRK immunoreactivity patterns in 2,669 solid tumors (comprising carcinomas, sarcomas, and melanocytic lesions) were retrospectively collected by nine laboratories and comprised tumor type, percentage of pan-TRK-positive tumor cells, staining intensity, cytoplasmic, membrane and/or nuclear staining pattern, and the presence or absence of NTRK fusion. Results: Overall, 2,457 tumors (92%) were pan-TRK negative and 212 neoplasms (8%) were pan-TRK positive. Twenty-two pan-TRK-positive tumors (0.8%) harbored an NTRK fusion, representing 10% of all pan-TRK-positive tumors. Cytoplasmic immunoreactivity was most often observed, followed by membrane immunoreactivity. Nuclear pan-TRK positivity was least frequent, but was most often (33%) associated with NTRK fusion. Conclusion: Pan-TRK IHC can be used to screen for NTRK fusions, especially in commonly diagnosed solid tumors with low NTRK fusion prevalence. In case of pan-TRK immunoreactivity, regardless of its intensity and tumor cell percentage, subsequent molecular tests should be performed to formally confirm the presence or absence of NTRK fusions. |
| Databáze: | OpenAIRE |
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