Effects of the methanol root extract of Carpolobia lutea on sperm indices, acrosome reaction, and sperm DNA integrity in cadmium-induced reproductive toxicity in male Wistar rats
| Autor: | Ooreoluwapo Ololade Daramola, Adeniran Oluwadamilare Akinola, Yinusa Raji, Adekunle Wahab Oyeyemi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Carpolobia lutea root extract
Male cadmium medicine.medical_treatment Acrosome reaction chemistry.chemical_element medicine.disease_cause Antioxidants Andrology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Testis medicine Animals Rats Wistar Saline Cadmium 030219 obstetrics & reproductive medicine Sperm Count Plant Extracts Acrosome Reaction Free radical scavenger Sperm Spermatozoa Rats Semen Analysis Oxidative Stress chemistry sperm DNA 030220 oncology & carcinogenesis sperm analysis Sperm Motility Original Article Methanol Reproductive toxicity Oxidative stress |
| Zdroj: | JBRA Assisted Reproduction |
| ISSN: | 1518-0557 1517-5693 |
| Popis: | Objective: Oxidative stress is a mechanism of cadmium-induced reproductive dysfunction. Carpolobia lutea is a free radical scavenger. Our study investigated the potential protective effects of Carpolobia lutea root methanol extract against cadmium-induced reproductive toxicity. Methods: We obtained the Carpolobia lutea root in Akure, and it was authenticated at the Forestry Research Institute of Nigeria (FRIN) herbarium, Ibadan, Nigeria, with FHI number 109784. We used Soxhlet extraction to obtain its methanol extract. We used thirty male Wistar rats (150-170g) in this study, (n=5 per group), and treated them as follows: Control (1 ml/kg normal saline), Cd (2 mg/kg), Cd+MCL (2 mg/kg+100 mg/kg), Cd+MCL (2 mg/kg+200 mg/kg), MCL (100 mg/kg), MCL (200 mg/kg). We administered Carpolobia lutea orally for 8 weeks. We administered a single dose of 2 mg/kg of cadmium intraperitoneally. We assessed the sperm profile using a computer-aided sperm analyzer. Under microscopy, we determined the sperm acrosome reaction and the DNA damage. We measured the seminal fructose level using spectrophotometry, and the data were analyzed using ANOVA at p |
| Databáze: | OpenAIRE |
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