Ecdysteroid receptors and their applications in agriculture and medicine
| Autor: | Markus Lezzi, Uwe Schlattner, Robert Eugene Hormann, Subba Reddy Palli |
|---|---|
| Přispěvatelé: | Hamant, Sarah, Department of Entomology [Lexington], University of Kentucky (UK), RheoGene Inc., Laboratoire de bioénergétique fondamentale et appliquée (LBFA), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), Department of Biology, Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology [Zürich] (ETH Zürich)-Institute of Cell Biology, University of Kentucky |
| Rok vydání: | 2006 |
| Předmět: |
MESH: Signal Transduction
Insecticides Receptors Steroid Insecta Coenzymes Genes Insect MESH: Genes Insect Ligands Transactivation MESH: Protein Structure Tertiary 0302 clinical medicine MESH: Insects MESH: Gene Expression Regulation Developmental MESH: Ligands MESH: Animals 0303 health sciences MESH: Coenzymes Gene Expression Regulation Developmental Agriculture MESH: Transcription Factors 3. Good health Medicine Signal transduction Functional genomics Dimerization hormones hormone substitutes and hormone antagonists MESH: Agriculture Signal Transduction MESH: Mutation Nanotechnology MESH: Ecdysteroids Computational biology Retinoid X receptor Biology Domain (software engineering) 03 medical and health sciences [SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular Biology Animals [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Transcription factor 030304 developmental biology Ecdysteroids MESH: Medicine DNA-binding domain MESH: Insecticides Protein Structure Tertiary Nuclear receptor MESH: Dimerization Mutation 030217 neurology & neurosurgery MESH: Receptors Steroid Transcription Factors |
| Zdroj: | Vitamins and hormones Vitamins and hormones, 2005, 73, pp.59-100. ⟨10.1016/S0083-6729(05)73003-X⟩ Vitamins and hormones, Elsevier, 2005, 73, pp.59-100. ⟨10.1016/S0083-6729(05)73003-X⟩ |
| ISSN: | 0083-6729 |
| DOI: | 10.1016/S0083-6729(05)73003-X⟩ |
| Popis: | International audience; This chapter summarizes the present knowledge on the ecdysteroid receptor (EcR) structure and function with special emphasis on those aspects that are relevant for gene switch applications, and describes gene switch technology with a focus on EcR‐based gene switches. The EcR is a member of the nuclear receptor superfamily and exhibits the typical modular structure composed of the N‐terminal A/B domain, the DNA‐binding C domain, the hinge (D) region, the ligand‐binding E domain, and the C-terminal F domain. The ligand‐binding domain is multifunctional and includes ligand‐dependent dimerization and transactivation functions, while ligand‐independent transactivation and dimerization functions are found in the terminal domains and in the region spanning the DNA binding domain and the N‐terminal region of the hinge, respectively. The EcR heterodimerizes with other members of the nuclear receptor superfamily noticeably with the ultraspiracle protein (USP), which is an orthologue of the vertebrate retinoic acid X receptor (RXR). The progress in made in EcR research has shown that the ligand‐binding pocket of EcR is highly flexible and adaptable. This property of EcR permits the development of receptor systems for practical application in medicine and agriculture. The application of EcR‐based gene switch technology in cellular systems toward functional genomics, drug and ligand discovery, and therapeutic protein production has already commenced grow rapidly in near future. The application of EcR‐based gene switches in plants has a great potential and benefits greatly from the fact that several potential ligands represent registered insecticides and have a track record of environmental safety. |
| Databáze: | OpenAIRE |
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