Co-Regulation of Cell Polarization and Migration by Caveolar Proteins PTRF/Cavin-1 and Caveolin-1
| Autor: | Rachael Barry, Debra Black, John F. Hancock, Fiona Simpson, Dorothy Loo, Noor Huda Daud, Samantha Murphy, Sally Martin, Libin Liu, C. S. Aung, Robert G. Parton, Michelle M. Hill, Paul F. Pilch, Marie-Odile Parat |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
rac1 GTP-Binding Protein
Science Blotting Western Caveolin 1 RAC1 Biology Biochemistry Metastasis 03 medical and health sciences Mice 0302 clinical medicine PTRF Cell Movement Caveolae Cell polarity Molecular Cell Biology Basic Cancer Research Animals 030304 developmental biology 0303 health sciences Multidisciplinary Microscopy Video Prostate Cancer Chemotaxis Neuropeptides Proteins Cancers and Neoplasms Cell Polarity Membrane Proteins RNA-Binding Proteins Cell migration Cellular Structures Cell biology rac GTP-Binding Proteins Rac GTP-Binding Proteins Genitourinary Tract Tumors Subcellular Organelles Oncology Microscopy Fluorescence 030220 oncology & carcinogenesis NIH 3T3 Cells Medicine Membranes and Sorting Cavin Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 7, Iss 8, p e43041 (2012) |
| ISSN: | 1932-6203 |
| Popis: | Caveolin-1 and caveolae are differentially polarized in migrating cells in various models, and caveolin-1 expression has been shown to quantitatively modulate cell migration. PTRF/cavin-1 is a cytoplasmic protein now established to be also necessary for caveola formation. Here we tested the effect of PTRF expression on cell migration. Using fluorescence imaging, quantitative proteomics, and cell migration assays we show that PTRF/cavin-1 modulates cellular polarization, and the subcellular localization of Rac1 and caveolin-1 in migrating cells as well as PKCα caveola recruitment. PTRF/cavin-1 quantitatively reduced cell migration, and induced mesenchymal epithelial reversion. Similar to caveolin-1, the polarization of PTRF/cavin-1 was dependent on the migration mode. By selectively manipulating PTRF/cavin-1 and caveolin-1 expression (and therefore caveola formation) in multiple cell systems, we unveil caveola-independent functions for both proteins in cell migration. |
| Databáze: | OpenAIRE |
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