A toolbox for the comprehensive analysis of small volume human intestinal samples that can be used with gastrointestinal sampling capsules
| Autor: | Barbara M. Bakker, Christiane Rösch, Theo Boer, Dirk-Jan Reijngoud, Albert Gerding, Guido J. E. J. Hooiveld, Melany Rios-Morales, M. Rebecca Heiner-Fokkema, Henk A. Schols, Ran An, Martijn Koehorst, Mara P H van Trijp, Naomi de Ruiter |
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| Přispěvatelé: | Center for Liver, Digestive and Metabolic Diseases (CLDM), AIMMS, Molecular Cell Physiology |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Dietary Fiber
Male 0301 basic medicine Workflow Dietary carbohydrates Feces Voeding Metabolisme en Genomica chemistry.chemical_compound 0302 clinical medicine Microbiologie RNA Ribosomal 16S Intestine Small Multidisciplinary Food Chemistry Ileostomy Gastroenterology Metabolism and Genomics Metabolisme en Genomica Medicine Female Nutrition Metabolism and Genomics 030211 gastroenterology & hepatology Composition (visual arts) Digestion DNA Bacterial Science Food chemistry DNA Ribosomal Microbiology Article Specimen Handling 03 medical and health sciences Voeding Levensmiddelenchemie Metabolomics Humans Life Science MolEco Nutrition VLAG Chromatography Quenching (fluorescence) Bacteria Fatty Acids Volatile Gastrointestinal Microbiome 030104 developmental biology chemistry Reagent Fermentation Urea Degradation (geology) |
| Zdroj: | Scientific Reports, 11(1):8133. Nature Publishing Group Scientific Reports, Vol 11, Iss 1, Pp 1-14 (2021) Scientific Reports, 11(1) Scientific Reports 11 (2021) 1 Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-021-86980-y |
| Popis: | Detailed knowledge on the fate of dietary components inside the human intestinal tract is lacking. Access to this inner world of digestion is now possible through novel human gastrointestinal sampling capsules. Due to the novelty of such devices, no methodology has been published to stabilise and analyse the resulting samples. A complicating factor is that excretion of such capsules in faeces may take days, while degradation of the dietary components continues. Therefore a stabilising reagent should be pre-loaded in the capsule to ensure the measurement of a representative sample. Considering the small volume of recovered samples, analytical methods must be optimized to collect as many data as possible from little material. We present a complete workflow for stabilising and analysing the fermentation status of dietary fibres in such samples, including microbiota, fibre degradation, and short chain fatty acids. The final quenching reagent was designed based on safety and effectiveness to inhibit fructo- and galacto-oligosaccharides degradation and short chain fatty acids production by human ileostomy microbiota, and subsequently validated in faecal samples. The final composition of the stock quenching reagent is 175 mM Tris, 525 mM NaCl, 35 mM EDTA, 12% SDS, and 8 M urea at pH 8.5. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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