Shotgun proteome analysis of beer and the immunogenic potential of beer polypeptides
| Autor: | Chiara Nitride, Alessandra Camarca, Francesco Addeo, Immacolata Vocca, Gianluca Picariello, Pasquale Ferranti, Carmen Gianfrani, Gianfranco Mamone |
|---|---|
| Přispěvatelé: | G., Picariello, G., Mamone, Nitride, Chiara, Addeo, Francesco, A., Camarca, I., Vocca, C., Gianfrani, Ferranti, Pasquale |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Adult
polypeptide Spectrometry Mass Electrospray Ionization Saccharomyces cerevisiae Proteins Adolescent Glutens Proteome T-Lymphocytes Molecular Sequence Data Biophysics Dot blot Biology Biochemistry Saccharomyces Humans Amino Acid Sequence Shotgun proteomics Child Peptide sequence Chromatography High Pressure Liquid mass spectrometry Plant Proteins business.industry food and beverages Beer Hordeum Allergens biology.organism_classification Celiac Disease Child Preschool Brewing Hordeum vulgare business Peptides allergen Prolamins |
| Zdroj: | Journal of proteomics 75 (2012): 5872–5882. info:cnr-pdr/source/autori:Picariello G, Mamone G, Nitride C, Addeo F, Camarca A, Vocca I, Gianfrani C, Ferranti P./titolo:Shotgun proteome analysis of beer and the immunogenic potential of beer polypeptides/doi:/rivista:Journal of proteomics (Print)/anno:2012/pagina_da:5872/pagina_a:5882/intervallo_pagine:5872–5882/volume:75 |
| Popis: | The majority of beer proteins originate from barley (Hordeum vulgare) which is used for brewing. Barley is known to contain celiacogenic gliadin-like prolamins (hordeins) along with other immunogenic proteins which endure malt proteases and the harsh conditions of brewing. In addition, a multitude of peptides that may retain or even amplify the immune-stimulating potential is released in beer because of proteolysis. The comprehensive annotation of the beer proteome is challenged both by the high concentration range of the protein entities and by a severe degree of processing-induced modifications. Overcoming the pitfalls of the classical two-dimensional electrophoresis approach coupled to mass spectrometry (MS), the gel-free shotgun proteomic analysis expanded the current inventory of a popular Italian beer to 33 gene products, including traces of intact B- and D-hordeins and 10 proteins from Saccharomyces spp. The high performance liquid chromatography-electrospray MS/MS peptidomic analysis of the low-molecular weight beer components disclosed a panel of hordein-derived peptides that encrypt gluten-like sequence motifs, potentially harmful to celiacs. The presence of antigliadin IgA-immunoresponsive prolamins was assayed by Western and dot blot using sera of N=4 celiac patients. Gliadin-reactive T-cell lines isolated from the intestine of N=5 celiacs activated an IFN-γ response when challenged with deamidated beer polypeptides. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|