Usefulness of a novel device to divide core needle biopsy specimens in a spatially matched fashion
| Autor: | Akihisa Ueno, Keisuke Tsuji, Takashi Ueda, Takumi Shiraishi, Yumiko Saito, Osamu Ukimura, Hidefumi Taniguchi, Yasuhiro Yamada, Takeshi Yamada, Masatomo Kaneko, Yuta Inoue, Fumiya Hongo, Atsuko Fujihara, Saya Ueda, Tsuyoshi Iwata, Toru Matsugasumi, Hideto Taga, Shogo Inui |
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| Rok vydání: | 2020 |
| Předmět: |
Core needle
Pathology medicine.medical_specialty lcsh:Medicine Diseases Mice SCID Real-Time Polymerase Chain Reaction Article Specimen Handling Mice Mouse xenograft Cell Line Tumor Biopsy medicine Animals Humans New device Genetic Testing lcsh:Science Experimental surgery Tumor xenograft Cancer Multidisciplinary medicine.diagnostic_test business.industry Biopsy Needle lcsh:R Neoplasms Experimental Needle biopsy Feasibility Studies lcsh:Q business Biomedical engineering Neoplasm Transplantation |
| Zdroj: | Scientific Reports, Vol 10, Iss 1, Pp 1-8 (2020) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | We developed a novel dividing device that can split needle biopsy tissues along longitude axis aiming to achieve definitive molecular-biological and genetical analysis with reference of pathological diagnosis of the side-by-side divided tissue as spatially matched information. The aim of this study was to evaluate the feasibility and potential usefulness of the novel dividing device to provide the appropriate materials for molecular diagnosis. The new device was examined using mouse xenograft tumors. Real-time quantitative PCR and genetic test were performed to evaluate the feasibility and usefulness of the device. All the samples from needle biopsy were successfully divided into two pieces. Quality and quantity from divided samples harbor high enough to perform gene expression analysis (real-time PCR) and genetic test. Using two divided samples obtained from xenograft tumor model by needle biopsy, the % length of xenograft tumor (human origin) was significantly correlated with the % human genomic DNA (p = 0.00000608, r = 0.987), indicating that these divided samples were spatially matched. The novel longitudinally dividing device of a needle biopsy tissue was useful to provide the appropriate materials for molecular-biological and genetical analysis with reference of pathological diagnosis as spatially matched information. |
| Databáze: | OpenAIRE |
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