Exclusive induction of G:C to A:T transitions by 3-azido-1,2-propanediol in yeast
| Autor: | Yukio Usui, Akira Sassa, Hiroyuki Yamagishi, Petr Grúz, Akemi Hosoda, Masatomi Shimizu |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Azides
Saccharomyces cerevisiae Proteins Health Toxicology and Mutagenesis Auxotrophy DNA Mutational Analysis Saccharomyces cerevisiae Mutagen medicine.disease_cause Canavanine chemistry.chemical_compound Drug Resistance Fungal Genetics medicine Mutation Dose-Response Relationship Drug biology fungi Mutagenesis Tryptophan food and beverages biology.organism_classification Yeast chemistry Biochemistry Propylene Glycols Amino Acid Transport Systems Basic Sodium azide |
| Zdroj: | Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 760:73-76 |
| ISSN: | 1383-5718 |
| DOI: | 10.1016/j.mrgentox.2013.10.006 |
| Popis: | Sodium azide is a strong mutagen which has been successfully employed in mutation breeding of crop plants. In biological systems, it is metabolized to azidoalanine, but further bioactivation to a putative ultimate mutagen as well as the nature of the induced DNA modifications leading to mutations remain elusive. In this study, mutations induced in the CAN1 gene of yeast Saccharomyces cerevisiae by the representative mutagen 3-azido-1,2-propanediol (azidoglycerol, AZG) have been sequenced. Analysis of the forward mutation spectrum to canavanine resistance revealed that AZG induced nearly exclusively G:C to A:T transitions. AZG also induced reversions to tryptophan prototrophy by base-pair substitutions in a dose-dependent manner. This unusual mutational specificity may be shared by other organic azido compounds. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect