Emerging vesiculo-type virus infections of freshwater fishes in Europe

Autor: A. M. Betts, Corinne Torhy, P. de Kinkelin, K. Way, D. M. Stone, Stefan Chilmonczyk, Abdenour Benmansour
Přispěvatelé: Centre for Environment, Fisheries and Aquaculture Science (CEFAS), Unité de recherche Virologie et Immunologie Moléculaires (VIM (UR 0892)), Institut National de la Recherche Agronomique (INRA), ProdInra, Migration
Rok vydání: 2003
Předmět:
[SDV]Life Sciences [q-bio]
viruses
Fluorescent Antibody Technique
Fresh Water
Fish Diseases
THYMALLIDS
Bass (fish)
Cluster Analysis
L GENE
Cells
Cultured
Phylogeny
0303 health sciences
Perch
Reverse Transcriptase Polymerase Chain Reaction
04 agricultural and veterinary sciences
Thymallus
PERCIDS
[SDV] Life Sciences [q-bio]
Europe
Trout
Electrophoresis
Polyacrylamide Gel
CENTRARCHIDS
food.ingredient
PROTEINS
Stizostedion
Molecular Sequence Data
Aquatic Science
Biology
Virus
03 medical and health sciences
food
FISH
Rhabdoviridae Infections
Animals
Ecology
Evolution
Behavior and Systematics
DNA Primers
030304 developmental biology
ANTIQENICITY
Base Sequence
Sequence Analysis
DNA
Vesiculovirus
Rhabdoviridae
biology.organism_classification
Virology
Perciformes
RHABDOVIRUS
Microscopy
Electron
Novel virus
040102 fisheries
0401 agriculture
forestry
and fisheries
Zdroj: Diseases of Aquatic Organisms
Diseases of Aquatic Organisms, Inter Research, 2003, 57 (3), pp.201-212
ISSN: 1616-1580
0177-5103
DOI: 10.3354/dao057201
Popis: International audience; Rhabdoviruses were isolated from perch Perca fluviatilis and largemouth bass À,fi'croptertts salmoides exhibiting clinical signs of disease, Preliminary studies indicated that these viruses could be neutralised by antisera to perch rhabdovirus (Dorson et al. 1984) and may be similar to those previotrsiy isolated from grayling Thymallus thymallus and pike-perch Stizostedion stizostedion.The relationship between these viruses and the previously characterised fish rhabdoviruses, pike fry rhabdovirus (PFRV), spring viraemia of carp virus (SVCV) and lake trout rhabdovirus, was investi- Eated. Viruses were propagated in bluegill fry (BF-2) cells and were characterised using electron microscopy, serum neutralisation tests, immunofluorescence tests, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and nucleotide sequence analysis. The bullet-shaped viral particles appeared to be compact, with spikes visible at the surface, a morphology similar to that of the vesiculovirus group of rhabdoviruses. Serum neutralisation tests showed that the viruses were antigenically closely related to the previously characterised perch rhabdovirus, but were not significantly neutralised by antisera to PFRV, SVCV or viral haemorrhagic septicaemia virus (VHSV), In immunofluorescence tests with perch rhabdovirus antisera, strong specific fluorescence was observed in cell cultures infected with the new rhabdovirus isolates, but no fluorescence was observed with antisera to PFRV, SVCV or VHSV. SDS-PAGE analysis reveaied a polypeptide profile typical of vesiculoviruses, but the novel virus isolates had different relative mobilities of their P and M proteins compared to PFRV and SVCV. Nucleotide sequence analysis was carried out using reverse transcriptase-polymerase chain reaction (RT-PCR) and DNA sequencing of a 439 base-pair region of the viral L gene. The novel rhabdovirus isolates had 95o/" identity to perch rh.abdovirus. Phylogenetic analysis using both maximum parsimony and neighbour-joining methods assigned the perch rhaboviruses to a separate group to that of PFRV, SVCV and lake trout rhabdovirus, These data are the initial characterisation of a group of emerging fish vesiculo-type viruses that are biochemically and geneticaliy distinct from the PFRV, SVCV and lake trout rhabdoviruses.
Databáze: OpenAIRE
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