Nonstimulatory peptides contribute to antigen-induced CD8-T cell receptor interaction at the immunological synapse
| Autor: | Tomasz Zal, Pia P. Yachi, Jeanette Ampudia, Nicholas R. J. Gascoigne |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Cell signaling
Time Factors CD3 Complex T cell CD8 Antigens Recombinant Fusion Proteins Immunology Amino Acid Motifs Green Fluorescent Proteins Immunoblotting Receptors Antigen T-Cell Down-Regulation chemical and pharmacologic phenomena Cell Communication Biology CD8-Positive T-Lymphocytes Major histocompatibility complex Article Immunological synapse Antigen Bacterial Proteins medicine Fluorescence Resonance Energy Transfer Immunology and Allergy Cytotoxic T cell Humans Immunoprecipitation Antigens Hybridomas Dose-Response Relationship Drug T-cell receptor Flow Cytometry Cell biology Luminescent Proteins medicine.anatomical_structure Microscopy Fluorescence biology.protein Tyrosine Peptides CD8 Algorithms Protein Binding |
| Zdroj: | Nature immunology. 6(8) |
| ISSN: | 1529-2908 |
| Popis: | It is unclear if the interaction between CD8 and the T cell receptor (TCR)-CD3 complex is constitutive or antigen induced. Here, fluorescence resonance energy transfer microscopy between fluorescent chimeras of CD3zeta and CD8beta showed that this interaction was induced by antigen recognition in the immunological synapse. Nonstimulatory endogenous or exogenous peptides presented simultaneously with antigenic peptides increased the CD8-TCR interaction. This finding indicates that the interaction between the intracellular regions of a TCR-CD3 complex recognizing its cognate peptide-major histocompatibility complex (MHC) antigen, and CD8 (plus the kinase Lck), is enhanced by a noncognate CD8-MHC interaction. Thus, the interaction of CD8 with a nonstimulatory peptide-MHC complex helps mediate T cell recognition of antigen, improving the coreceptor function of CD8. |
| Databáze: | OpenAIRE |
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