Erythroferrone as a sensitive biomarker to detect stimulation of erythropoiesis
| Autor: | Jean-Daniel Tissot, Emeric Gottardo, Kelvin Ramirez Cuevas, Nathan E. Townsend, Bernard Favrat, Tiia Kuuranne, Nicolas Leuenberger, Céline Schobinger, Sven Christian Voss |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Adult
Male medicine.medical_specialty Iron Peptide Hormones Pharmaceutical Science Stimulation 01 natural sciences Analytical Chemistry Injections 03 medical and health sciences Young Adult 0302 clinical medicine Blood doping Hepcidin Internal medicine medicine Biomarkers/blood Erythropoiesis/drug effects Erythropoietin/administration & dosage Erythropoietin/pharmacology Exercise Hematinics/administration & dosage Hematinics/pharmacology Humans Iron/administration & dosage Iron/pharmacology Peptide Hormones/blood Peptides/administration & dosage Peptides/pharmacology Substance Abuse Detection/methods blood doping erythroferrone immunoassay Environmental Chemistry Erythropoiesis 030216 legal & forensic medicine Erythropoietin Spectroscopy biology Chemistry 010401 analytical chemistry fungi food and beverages Erythroferrone 0104 chemical sciences Substance Abuse Detection Endocrinology biology.protein Hematinics Creatine kinase Peptides Biomarkers Hormone medicine.drug |
| Zdroj: | Drug testing and analysis, vol. 12, no. 2, pp. 261-267 |
| Popis: | Erythroferrone (ERFE) is a glycoprotein hormone secreted by erythroblasts in response to erythropoietin stimulation. ERFE suppresses the hepatic synthesis of the master iron-regulatory hormone, hepcidin. The impact of erythropoiesis stimulation on ERFE secretion in humans is poorly understood. This paucity of information is due in part to the lack of available means for ERFE quantification in serum samples. The present study tested a new sensitive sandwich immunoassay for human ERFE. This assay was used to demonstrate that injection of various erythropoiesis stimulating agents (ESAs) increased the blood ERFE levels in healthy volunteers. After exogenous stimulation of erythropoiesis, ERFE increased up to 8-fold with a detection window of 13 days. The impact of one unit of blood withdrawal on erythropoiesis stimulation of ERFE was also tested. ERFE significantly increased after blood withdrawal in subjects injected with both iron and saline solution, suggesting that iron supplementation did not mask the ERFE increase after blood withdrawal. The effects of exercise-induced muscle damage on ERFE was assessed by comparing ERFE levels with creatine kinase levels in samples from subjects with heavy exercise loads, and determined that this was not a confounder. The ERFE assay is a sensitive means to investigate the connection between iron metabolism and erythropoiesis in humans, and to detect ESA abuse in the antidoping field. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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