Trans-repressor activity of nuclear glycosaminoglycans on Fos and Jun/AP-1 oncoprotein-mediated transcription
| Autor: | A D Cardin, G A Martin, S J Busch, Richard L. Jackson, M Mano, Roger L. Barnhart |
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| Rok vydání: | 1992 |
| Předmět: |
Chloramphenicol O-Acetyltransferase
Male Cell cycle checkpoint Transcription Genetic Proto-Oncogene Proteins c-jun Aorta Thoracic Biology Transfection Muscle Smooth Vascular Cell Line Suppression Genetic Genes jun Gene expression medicine Transcriptional regulation Animals Humans RNA Messenger Cells Cultured Glycosaminoglycans Cell Nucleus Cell growth Heparin Cell Cycle Genes fos Rats Inbred Strains Cell Biology Articles Cell cycle Molecular biology Rats Cell nucleus medicine.anatomical_structure Cell culture Tetradecanoylphorbol Acetate Proto-Oncogene Proteins c-fos HeLa Cells Transcription Factors |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 0021-9525 |
| Popis: | Heparin blocks the phorbol ester-induced progression of nontransformed cells through the G0/G1 phase (Wright, T.C., L.A. Pukac, J.J. Castellot, M.J. Karnovsky, R.A. Levine, H.-Y. Kim-Park, and J. Campisi. 1989. Proc. Natl. Acad. Sci. USA. 86: 3199-3203) or G1 to S phase (Reilly, C. F., M. S. Kindy, K. E. Brown, R. D. Rosenberg, and G. E Sonenshein. 1989. J. Biol. Chem. 264:6990-6995) of the cell cycle. Cell cycle arrest was associated with decreased levels of stage-specific mRNAs suggesting transcriptional regulation of cell growth. In the present report, we show that heparin selectively repressed TPA-inducible AP-1-mediated gene expression. Heparin-induced trans-repression was observed in primary vascular smooth muscle cells, as well as in the transformed HeLa cell line and in nondifferentiated F9 teratocarcinoma cells. Inhibition of AP-1-mediated trans-activation occurred with heparin and pentosan polysulfate but not with chondroitin sulfate A or C. Heparin-binding peptides or heparitinase I addition to nuclear lysates of heparin-treated cells allowed enhanced recovery of endogenous AP-1-specific DNA binding activity. We propose a model in which nuclear glycosaminoglycans play a trans-regulatory role in altering the patterns of inducible gene expression. |
| Databáze: | OpenAIRE |
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