The effects of voluntary complex and regular wheel running exercises on the levels of 8‐oxoguanine DNA glycosylase, semaphorin 3B, H2O2, and apoptosis in the hippocampus of diabetic rats

Autor: Ziya Fallah Mohammadi, Hossein Falah Mohammadi, Mohammad Fazelzadeh, Mohammad Esmaeil Afzalpour
Rok vydání: 2021
Předmět:
Male
medicine.medical_specialty
Programmed cell death
Guanine
medicine.medical_treatment
Intraperitoneal injection
Semaphorins
Motor Activity
Hippocampus
050105 experimental psychology
lcsh:RC321-571
DNA Glycosylases
Diabetes Mellitus
Experimental
03 medical and health sciences
Behavioral Neuroscience
0302 clinical medicine
Semaphorin
Diabetes mellitus
Internal medicine
medicine
Animals
Hippocampus (mythology)
0501 psychology and cognitive sciences
Rats
Wistar
lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry
OGG1
Original Research
business.industry
semaphorin 3B
05 social sciences
apoptosis
Hydrogen Peroxide
medicine.disease
Streptozotocin
Rats
Endocrinology
Apoptosis
voluntary exercise
complex wheel running
Analysis of variance
business
030217 neurology & neurosurgery
medicine.drug
Zdroj: Brain and Behavior
Brain and Behavior, Vol 11, Iss 3, Pp n/a-n/a (2021)
ISSN: 2162-3279
Popis: Purpose One of the most frequent complications associated with diabetes mellitus is apoptosis within the brain which can lead to cognitive disorders. Exercise is considered the best non‐pharmacological approach to reduce the severity and extent of cell death through poorly‐understood mechanisms. The aim of this study was to investigate the effects of voluntary complex and regular wheel running on the levels of 8‐oxoguanine DNA glycosylase (OGG1), semaphorin 3B (sema3B), hydrogen peroxide (H2O2), and apoptosis in the hippocampus of diabetic rats. Methods 48 Wistar male rats were randomly divided into 6 groups: healthy control (C), diabetes control (D), regular wheel running + diabetes (RWD), complex wheel running + diabetes (CWD), healthy regular wheel running (RW), and healthy complex wheel running (CW). The diabetic rat model was produced by intraperitoneal injection of streptozotocin (STZ). The protocol encompassed a 4‐week voluntary running training regimen on regular and complex wheel running apparatus. The rats were sacrificed 48 hr after the last training session. To measure the protein concentrations within the hippocampus, ELISA has been utilized. One‐way ANOVA was used to compare the groups. Results There were no significant differences in OGG1 protein levels between the groups. H2O2 level in the D group was significantly higher than the C group (p = .002), while this in RWD and CWD groups was considerably lower than the D group (p = .002 and p = .003, respectively). In the D group, the levels of apoptosis and Sema3B were significantly (p = .001 and p = .007, respectively) higher than C, RWD (p = .001, p = .0001, respectively), and CWD groups (p = .001, p = .006, respectively). Nevertheless, there were not any significant differences between RWD and CWD groups. Conclusion The increased levels of Sema3B, H2O2, and apoptosis within the hippocampus associated with diabetes could be noticeably restored by both types of voluntary wheel running protocols.
Performing both complex and voluntary wheel running protocols enhanced apoptotic indices in diabetic rat model. The increased levels of Sema3B, H2O2, and apoptosis within hippocampus associated with diabetes could be noticeably restored by both types of exercises. Therefore, exercise can be used as a safe therapeutic approach against negative impacts of diabetes on the brain.
Databáze: OpenAIRE
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