Heterodimerization of calcium sensing receptors with metabotropic glutamate receptors in neurons
| Autor: | Susan G. Wilt, Gerda E. Breitwieser, Lucio Gama |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Enzyme-Linked Immunosorbent Assay
Receptors Metabotropic Glutamate Transfection Biochemistry Hippocampus Cell Line Cerebellum Animals Humans Disulfides Molecular Biology Neurons Chemistry Metabotropic glutamate receptor 5 Metabotropic glutamate receptor 4 Metabotropic glutamate receptor 7 Calcium-Binding Proteins Metabotropic glutamate receptor 6 Brain Cell Biology Precipitin Tests Cell biology Rats nervous system Microscopy Fluorescence Metabotropic glutamate receptor Metabotropic glutamate receptor 1 Calcium Cattle Metabotropic glutamate receptor 3 Metabotropic glutamate receptor 2 human activities Dimerization Protein Binding |
| Zdroj: | The Journal of biological chemistry. 276(42) |
| ISSN: | 0021-9258 |
| Popis: | Calcium sensing (CaR) and Group I metabotropic glutamate receptors exhibit overlapping expression patterns in brain, and share common signal transduction pathways. To determine whether CaR and Group I metabotropic glutamate receptors (mGluRs) (mGluR1alpha and mGluR5) can form heterodimers, we immunoprecipitated CaR from bovine brain and observed co-precipitation of mGluR1alpha. CaR and mGluR1alpha co-localize in hippocampal and cerebellar neurons, but are expressed separately in other brain regions. In vitro transfection studies in HEK-293 cells established the specificity and disulfide-linked nature of the CaR:mGluR1alpha (CaR:mGluR5) interactions. CaR:mGluR1alpha (CaR:mGluR5) heterodimers exhibit altered trafficking via Homer 1c when compared with CaR:CaR homodimers. CaR becomes sensitive to glutamate-mediated internalization when present in CaR:mGluR1alpha heterodimers. These results demonstrate cross-family covalent heterodimerization of CaR with Group I mGluRs, and increase the potential role(s) for CaR in modulating neuronal function. |
| Databáze: | OpenAIRE |
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