Effects of Bothrops moojeni venom on Leishmania amazonensis promastigote forms
| Autor: | Francisco P. M. Oliveira, Napolitano, Cristiano Gonçalves Pereira, Patrícia de Castilhos, Maria Aparecida de Souza, Aln Silva |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
lcsh:Arctic medicine. Tropical medicine
lcsh:RC955-962 Venom Peptide Protein degradation Toxicology complex mixtures Nitric oxide Bothrops moojeni chemistry.chemical_compound lcsh:RA1190-1270 parasitic diseases lcsh:Zoology lcsh:QL1-991 lcsh:Toxicology. Poisons chemistry.chemical_classification biology Chemistry biology.organism_classification Leishmania Leishmania braziliensis Infectious Diseases Biochemistry Snake venom Immunology Animal Science and Zoology Parasitology |
| Zdroj: | Journal of Venomous Animals and Toxins including Tropical Diseases v.17 n.2 2011 The Journal of venomous animals and toxins including tropical diseases Universidade Estadual Paulista (UNESP) instacron:UNESP Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 17, Iss 2, Pp 150-158 (2011) Journal of Venomous Animals and Toxins including Tropical Diseases, Volume: 17, Issue: 2, Pages: 150-158, Published: 2011 Scopus-Elsevier |
| Popis: | In the present work, the effect of Bothrops moojeni venom fractions on Leishmania promastigotes was evaluated. The snake venom was fractionated into five protein fractions (E1 to E5), by ion exclusion chromatography, that were used to treat Leishmania amazonensis and Leishmania braziliensis promastigote forms whereas the viability and nitric oxide production were evaluated. It was observed that E5 venom fraction strongly inhibited Leishmania amazonensis nitric oxide production, while in Leishmania braziliensis the nitric oxide production was enhanced in all doses. Bothrops moojeni crude venom reduced the viability of both parasites in a dose-dependent manner and a peptide of 64 kDa was apparently degraded. Bothrops moojeni E5 venom fraction only reduced the viability and nitric oxide production of Leishmania amazonensis and no protein degradation was observed. Thus, these results suggest that Bothrops moojeni E5 venom fraction may offer components with a promising antileishmanial therapeutic application.Key words |
| Databáze: | OpenAIRE |
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