Protein Tpr is required for establishing nuclear pore-associated zones of heterochromatin exclusion
| Autor: | Sandra Krull, Sonja Reidenbach, Julia Dörries, Helene Norder, Johan Thyberg, Bjorn M. M. Boysen, Volker C. Cordes, Lars O. Magnius |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Euchromatin
Somatic cell Heterochromatin Tpr Biology Models Biological Article General Biochemistry Genetics and Molecular Biology Mice Prophase Microscopy Electron Transmission RNA interference nuclear pore complex Proto-Oncogene Proteins medicine Animals Humans Gene Silencing Nuclear pore Molecular Biology Cell Nucleus Microscopy Confocal General Immunology and Microbiology nuclear compartmentalization General Neuroscience heterochromatin Molecular biology Chromatin Cell biology Nuclear Pore Complex Proteins Poliovirus Cell nucleus medicine.anatomical_structure Microscopy Fluorescence RNA Interference HeLa Cells |
| Zdroj: | The EMBO Journal EMBO Journal |
| ISSN: | 1460-2075 0261-4189 |
| DOI: | 10.1038/emboj.2010.54 |
| Popis: | Amassments of heterochromatin in somatic cells occur in close contact with the nuclear envelope (NE) but are gapped by channel- and cone-like zones that appear largely free of heterochromatin and associated with the nuclear pore complexes (NPCs). To identify proteins involved in forming such heterochromatin exclusion zones (HEZs), we used a cell culture model in which chromatin condensation induced by poliovirus (PV) infection revealed HEZs resembling those in normal tissue cells. HEZ occurrence depended on the NPC-associated protein Tpr and its large coiled coil-forming domain. RNAi-mediated loss of Tpr allowed condensing chromatin to occur all along the NE's nuclear surface, resulting in HEZs no longer being established and NPCs covered by heterochromatin. These results assign a central function to Tpr as a determinant of perinuclear organization, with a direct role in forming a morphologically distinct nuclear sub-compartment and delimiting heterochromatin distribution. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text