Effect of Keishibukuryogan, a Japanese Traditional Kampo Prescription, on Improvement of Microcirculation and Oketsu and Induction of Endothelial Nitric Oxide: A Live Imaging Study
| Autor: | Tsutomu Tomita, Kazumasa Aoyagi, Hirofumi Matsui, Aki Hirayama |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Article Subject Kampo Hemodynamics Vasodilation Pharmacology Nitric oxide Microcirculation 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Live cell imaging Oral administration Medicine business.industry lcsh:Other systems of medicine lcsh:RZ201-999 030104 developmental biology Complementary and alternative medicine chemistry 030220 oncology & carcinogenesis business Keishibukuryogan Research Article |
| Zdroj: | Evidence-Based Complementary and Alternative Medicine, Vol 2017 (2017) Evidence-based Complementary and Alternative Medicine : eCAM |
| ISSN: | 1741-427X |
| DOI: | 10.1155/2017/3620130 |
| Popis: | Oketsu is a characteristic condition that plays an important role in Kampo, Japanese traditional medicine, and includes multiple aspects of hemodynamic disorders. This study aims to clarify the microcirculation of Oketsu and the pharmacological effect of Keishibukuryogan, an anti-Oketsu Kampo prescription, using live imaging techniques. Oral administration of Keishibukuryogan induced significant vasodilation of murine subcutaneous arterioles compared to the preadministration level. This vasodilatation peaked 60 min after administration and persisted for 90 min. The blood velocity in the subcutaneous capillary was also increased by Keishibukuryogan in generally the same manner. In rat mesenteric arterioles, Keishibukuryogan administration improved microhemodynamic parameters, including the resolution of erythrocyte congestion and the cell-free layer, which are representative of Oketsu pathology. Live imaging revealed an increase of diaminofluorescein-2 diacetate fluorescence, a nitric oxide (NO) specific reagent, in the arterial endothelium following Keishibukuryogan administration. This fluorescence was most remarkable at vascular bifurcations but was present throughout the mesenteric arterioles. This study demonstrates the successful imaging of Oketsu pathology with respect to microcirculation and the anti-Oketsu effects of Keishibukuryogan, namely, vasodilation of arterioles, increased blood velocity, and resolution of erythrocyte congestion. The anti-Oketsu effect of Keishibukuryogan is related to endothelial NO production. |
| Databáze: | OpenAIRE |
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