Urease is an essential component of the acid response network of Staphylococcus aureus and is required for a persistent murine kidney infection

Autor: McKenzie K. Lehman, Robert Powers, Vinai Chittezham Thomas, Paul D. Fey, Tammy Kielian, Sujata S. Chaudhari, Kelsey J. Yamada, Fatema Bhinderwala, Kirk W. Foster, Chunyi Zhou
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Male
Arginine
Urease
Staphylococcus
medicine.disease_cause
Pathology and Laboratory Medicine
Kidney
Biochemistry
chemistry.chemical_compound
Mice
Medicine and Health Sciences
Urea
Homeostasis
Staphylococcus Aureus
Amino Acids
Biology (General)
chemistry.chemical_classification
0303 health sciences
biology
Organic Compounds
Monosaccharides
Catabolism
Hydrogen-Ion Concentration
Staphylococcal Infections
Enzymes
Bacterial Pathogens
Chemistry
medicine.anatomical_structure
Staphylococcus aureus
Medical Microbiology
CCPA
Ureases
Physical Sciences
Female
Kidney Diseases
Pathogens
Anatomy
Basic Amino Acids
Research Article
Nitrogen
QH301-705.5
Immunology
Carbohydrates
Microbiology
03 medical and health sciences
Bacterial Proteins
Ammonia
Virology
Genetics
medicine
Animals
Molecular Biology
Microbial Pathogens
030304 developmental biology
Bacteria
030306 microbiology
Organic Chemistry
Chemical Compounds
Organisms
Biology and Life Sciences
Proteins
Kidneys
Renal System
RC581-607
Mice
Inbred C57BL
Enzyme
Glucose
Metabolism
chemistry
biology.protein
Enzymology
Parasitology
Immunologic diseases. Allergy
Acids
Zdroj: PLoS Pathogens, Vol 15, Iss 1, p e1007538 (2019)
PLoS Pathogens
ISSN: 1553-7374
1553-7366
Popis: Staphylococcus aureus causes acute and chronic infections resulting in significant morbidity. Urease, an enzyme that generates NH3 and CO2 from urea, is key to pH homeostasis in bacterial pathogens under acidic stress and nitrogen limitation. However, the function of urease in S. aureus niche colonization and nitrogen metabolism has not been extensively studied. We discovered that urease is essential for pH homeostasis and viability in urea-rich environments under weak acid stress. The regulation of urease transcription by CcpA, Agr, and CodY was identified in this study, implying a complex network that controls urease expression in response to changes in metabolic flux. In addition, it was determined that the endogenous urea derived from arginine is not a significant contributor to the intracellular nitrogen pool in non-acidic conditions. Furthermore, we found that during a murine chronic renal infection, urease facilitates S. aureus persistence by promoting bacterial fitness in the low-pH, urea-rich kidney. Overall, our study establishes that urease in S. aureus is not only a primary component of the acid response network but also an important factor required for persistent murine renal infections.
Author summary Urease has been reported to be crucial to bacteria in environmental adaptation, virulence, and defense against host immunity. Although the function of urease in S. aureus is not clear, recent evidence suggests that urease is important for acid resistance in various niches. Our study deciphered a function of S. aureus urease both in laboratory conditions and during host colonization. Furthermore, we uncovered the major components of the regulatory system that fine-tunes the expression of urease. Collectively, this study established the dual function of urease which serves as a significant part of the S. aureus acid response while also serving as an enzyme required for persistent kidney infections and potential subsequent staphylococcal metastasis.
Databáze: OpenAIRE
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