Ecology of antibiotic resistant coagulase-negative staphylococci isolated from the production chain of a typical Italian salami
| Autor: | Vincenza Pisacane, Edoardo Puglisi, Maria Luisa Callegari, Lorenzo Morelli, Annalisa Rebecchi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Staphylococcus pasteuri
biology Staphylococcus xylosus Kanamycin biology.organism_classification Microbiology Staphylococcus cohnii food safety Antibiotic resistance Staphylococcus epidermidis Staphylococcus hominis Settore AGR/16 - MICROBIOLOGIA AGRARIA medicine Coagulase Food Science Biotechnology medicine.drug |
| Popis: | Bacteria carrying antibiotic resistance (AR) genes may represent a hazard for human health. The objective of this study was to evaluate the relationship between the origin, cell number, biotype, phenotypic and genotypic AR profile of coagulase-negative staphylococci (CNS) isolated from the production chain of Salame Piacentino . The 390 isolates were genetically ascribed to 98 different strains belonging to 13 Staphylococcus species: Staphylococcus xylosus and Staphylococcus pasteuri were the predominant followed by Staphylococcus hominis , Staphylococcus cohnii and Staphylococcus epidermidis . Eight antibiotic resistant strains, isolated from skin and salami samples, were detected in different steps along the manufacturing process. Determination of the minimum inhibition concentrations of all CNS strains showed a high frequency of phenotypic tetracycline and erythromycin (83.7% and 68.4% respectively) and a lower kanamycin (40.8%) resistant. The most frequently detected AR genes were erm C (37.8%), tet (L) and tet (K) (31.6% and 34.7% respectively) mainly in skin before washing with water at 65 °C and in faeces samples, but only S. xylosus harbouring tet (K) gene was detected during the salami ripening. Our findings show that, the production chain of Salame Piacentino is a source of antibiotic resistant CNS, but the salami manufacturing process leads a strong reduction of the antibiotic resistant strains and the hazard for human health. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|