Decalpenic acid induces early osteoblastic markers in pluripotent mesenchymal cells via activation of retinoic acid receptor γ
| Autor: | Manabu Kawada, Yoshio Nishimura, Shuichi Sakamoto, Fukiko Kojima, Hayamitsu Adachi, Isao Momose |
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| Rok vydání: | 2012 |
| Předmět: |
Pluripotent Stem Cells
medicine.drug_class Receptors Retinoic Acid Biophysics Retinoic acid Carboxylic Acids Retinoic acid receptor beta Naphthalenes Biochemistry Retinoic acid-inducible orphan G protein-coupled receptor Cell Line chemistry.chemical_compound Mice Dibenzazepines medicine Animals Retinoid Gene Silencing Molecular Biology Osteoblasts Chemistry Gene Expression Profiling Cell Differentiation Mesenchymal Stem Cells Cell Biology Retinoic acid receptor gamma Retinoid X receptor gamma Molecular biology Retinoic acid receptor Retinoic acid receptor alpha Biomarkers |
| Zdroj: | Biochemical and biophysical research communications. 422(4) |
| ISSN: | 1090-2104 |
| Popis: | Decalpenic acid is a natural small molecule previously isolated from the fermentation broth of fungi that induces early osteoblastic markers in pluripotent mesenchymal cells. Treatment of mouse pluripotent mesenchymal C3H10T1/2 cells with decalpenic acid gave rise to a morphological change similar to that induced by the treatment with retinoic acid, i.e. the cells adopted a more elongated spindle shape. Using a retinoic acid response element reporter and receptor activity assays, we show that decalpenic acid is a new retinoid with selectivity towards retinoic acid receptors γ and α. The induction of early osteoblastic markers by decalpenic acid was significantly inhibited by treatment with the retinoid antagonist, LE540, or with small interfering RNA-mediated knockdown of retinoic acid receptor γ. These results demonstrated that decalpenic acid induces early osteoblastic markers in pluripotent mesenchymal cells through activation of retinoic acid receptor γ. |
| Databáze: | OpenAIRE |
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