Chemoattractants stimulate degradation of methylated phospholipids and release of arachidonic acid in rabbit leukocytes
| Autor: | Barbara A. Corcoran, Krishnamoorthy Venkatasubramanian, Fusao Hirata, Elliot Schiffmann, Julius Axelrod |
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| Rok vydání: | 1979 |
| Předmět: |
Arachidonic Acids
chemistry.chemical_compound Methionine Phospholipase A2 Phorbol Esters Leukocytes Animals Choline Phospholipids Unsaturated fatty acid N-Formylmethionine Multidisciplinary biology Chemotaxis Metabolism Chemotaxis Leukocyte Kinetics chemistry Biochemistry biology.protein lipids (amino acids peptides and proteins) Arachidonic acid Rabbits Oligopeptides Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 76:2640-2643 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.76.6.2640 |
| Popis: | When rabbit peritoneal leukocytes were treated with chemoattractants such as fMet-Leu-Phe, an apparent decrease of [3H]methyl incorporation into the lipid fraction from L-[methyl-3H]methionine was observed. This decrease was a result of increased degradation of methylated phospholipids, not of decreased synthesis. Chemotactic peptides did not affect the metabolism of the phospholipids in which [methyl-14C]choline was incorporated. The disappearance of the [3H]methyl group was associated with the release of [1-14C]arachidonic acid from phospholipids prelabeled with these compounds. These findings suggested the activation by chemoattractants of phospholipase A2, an enzyme that removes an unsaturated fatty acid from phospholipids. The order of potency of chemoattractants for the stimulated degradation of phospholipids was in good agreement with that for chemotaxis. Mepacrine (quinacrine) and hydrocortisone inhibited and a phorbol ester enhanced both chemotaxis and phospholipase A2 activity. These results, taken together, suggest close association of the metabolism of methylated phospholipids with chemotaxis in rabbit peritoneal leukocytes. |
| Databáze: | OpenAIRE |
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