Laser Photochemotherapy With Anthracyclines on Cultured Human Squamous Carcinoma Cells
| Autor: | Quinten M. Vanderwerf, Marcos B. Paiva, Phil-Sang Chung, Dan J. Castro, G. St. A. Letts, J. Soudant, Romaine E. Saxton |
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| Rok vydání: | 1996 |
| Předmět: |
Pathology
medicine.medical_specialty medicine.medical_treatment Tumor Cells Cultured Carcinoma medicine Humans Chemotherapy Antibiotics Antineoplastic Photosensitizing Agents biology business.industry Topoisomerase Daunorubicin Cancer medicine.disease Molecular biology In vitro Squamous carcinoma Microscopy Fluorescence Photochemotherapy Otorhinolaryngology Targeted drug delivery Cell culture Carcinoma Squamous Cell biology.protein Laser Therapy Drug Screening Assays Antitumor business |
| Zdroj: | The Laryngoscope. 106:257-262 |
| ISSN: | 1531-4995 0023-852X |
| DOI: | 10.1097/00005537-199603000-00003 |
| Popis: | A new treatment for cancer has been tested in vitro using light-sensitive anthracyclines followed by laser photoactivation, as described by several investigators. We previously reported 10-fold enhanced laser killing after 2 hours of incubation with daunomycin by cultured human carcinoma cells. This short-term uptake leads to drug localization in cytoplasmic and membrane sites prior to nuclear accumulation and topoisomerase inhibition. In the present study, daunomycin was incubated for 2 or 24 hours with P3 squamous carcinoma cells to directly compare cytoplasmic vs. nuclear drug targeting before and after KTP-532 laser activation. Monolayer cultures of the P3 cells sensitized with daunomycin for 2 hours, then chilled (4 degree C), and exposed to the KTP laser (532 nm, 94.2 J/cm2) had a 2- to 10-fold increased therapeutic response compared with drug or laser alone when measured by MTT tetrazolium assays. After 24 hours of incubation with daunomycin, the chemotherapeutic response of P3 tumor cells was amplified 2-fold by laser exposure. The results suggest that daunomycin and laser treatment can be combined for improved therapy of human cancer. |
| Databáze: | OpenAIRE |
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