Deregulation of Ca2+-Signaling Systems in White Adipocytes, Manifested as the Loss of Rhythmic Activity, Underlies the Development of Multiple Hormonal Resistance at Obesity and Type 2 Diabetes
| Autor: | Egor A. Turovsky, Maria V. Turovskaya, V. V. Dynnik |
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| Rok vydání: | 2021 |
| Předmět: |
Male
0301 basic medicine Periodicity NO and protein kinase G Adipocytes White White adipose tissue Mice 0302 clinical medicine Biology (General) Receptor Cells Cultured Spectroscopy Epididymis Chemistry Ryanodine receptor Palmitoylcarnitine General Medicine feedback control of Ca2+ signaling systems Computer Science Applications Acetylcholine medicine.drug medicine.medical_specialty Nitric Oxide Synthase Type III QH301-705.5 G protein Primary Cell Culture loss of rhythmicity and general hormonal resistance to obesity Peptide hormone Diet High-Fat Article Catalysis Diabetes Mellitus Experimental Inorganic Chemistry murine white adipocytes 03 medical and health sciences GTP-Binding Proteins Internal medicine medicine Animals Calcium Signaling Obesity Physical and Theoretical Chemistry QD1-999 Molecular Biology Cell Size Phospholipase C Ca2+ oscillations and triggering phenomena Organic Chemistry G proteins interplay Angiotensin II 030104 developmental biology Endocrinology Diabetes Mellitus Type 2 Type C Phospholipases 030217 neurology & neurosurgery |
| Zdroj: | International Journal of Molecular Sciences Volume 22 Issue 10 International Journal of Molecular Sciences, Vol 22, Iss 5109, p 5109 (2021) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms22105109 |
| Popis: | Various types of cells demonstrate ubiquitous rhythmicity registered as simple and complex Ca2+-oscillations, spikes, waves, and triggering phenomena mediated by G-protein and tyrosine kinase coupled receptors. Phospholipase C/IP3-receptors (PLC/IP3R) and endothelial NO-synthase/Ryanodine receptors (NOS/RyR)–dependent Ca2+ signaling systems, organized as multivariate positive feedback generators (PLC-G and NOS-G), underlie this rhythmicity. Loss of rhythmicity at obesity may indicate deregulation of these signaling systems. To issue the impact of cell size, receptors’ interplay, and obesity on the regulation of PLC-G and NOS-G, we applied fluorescent microscopy, immunochemical staining, and inhibitory analysis using cultured adipocytes of epididumal white adipose tissue of mice. Acetylcholine, norepinephrine, atrial natriuretic peptide, bradykinin, cholecystokinin, angiotensin II, and insulin evoked complex [Ca2+]i responses in adipocytes, implicating NOS-G or PLC-G. At low sub-threshold concentrations, acetylcholine and norepinephrine or acetylcholine and peptide hormones (in paired combinations) recruited NOS-G, based on G proteins subunits interplay and signaling amplification. Rhythmicity was cell size- dependent and disappeared in hypertrophied cells filled with lipids. Contrary to control cells, adipocytes of obese hyperglycemic and hypertensive mice, growing on glucose, did not accumulate lipids and demonstrated hormonal resistance being non responsive to any hormone applied. Preincubation of preadipocytes with palmitoyl-L-carnitine (100 nM) provided accumulation of lipids, increased expression and clustering of IP3R and RyR proteins, and partially restored hormonal sensitivity and rhythmicity (5–15% vs. 30–80% in control cells), while adipocytes of diabetic mice were not responsive at all. Here, we presented a detailed kinetic model of NOS-G and discussed its control. Collectively, we may suggest that universal mechanisms underlie loss of rhythmicity, Ca2+-signaling systems deregulation, and development of general hormonal resistance to obesity. |
| Databáze: | OpenAIRE |
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