Growth inhibition of pathogenic microorganisms by Pseudomonas protegens EMM-1 and partial characterization of inhibitory substances
| Autor: | Verónica Quintero-Hernández, Jesús Muñoz-Rojas, Paul Gaytán, Antonino Baez, América Paulina Rivera-Urbalejo, Alberto Aguayo-Acosta, Roberto Carlos Llano-Villarreal, María del Rocío Bustillos-Cristales, Víctor Rivelino Juárez-González, Catherine Cesa-Luna |
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| Rok vydání: | 2020 |
| Předmět: |
Plant Science
Pathology and Laboratory Medicine Toxicology Bacteriocins Anti-Infective Agents Drug Resistance Multiple Bacterial Medicine and Health Sciences Toxins Bacillus licheniformis Data Management 0303 health sciences Multidisciplinary Ecology biology Plant Bacterial Pathogens Chemistry Pseudomonas Eukaryota Phylogenetic Analysis Bacterial Infections Plants Antimicrobial Bacterial Pathogens Phylogenetics Experimental Organism Systems Medical Microbiology Rhizosphere Medicine Pathogens Research Article Computer and Information Sciences Science Toxic Agents Bacterial Toxins Microbacterium Plant Pathogens Microbial Sensitivity Tests Research and Analysis Methods Microbiology Zea mays 03 medical and health sciences Pseudomonas protegens Model Organisms Bacteriocin Plant and Algal Models Plant-Environment Interactions Antibiosis Evolutionary Systematics Grasses Microbial Pathogens Taxonomy Plant Diseases 030304 developmental biology Evolutionary Biology Bacteria 030306 microbiology Plant Ecology Ecology and Environmental Sciences Organisms Biology and Life Sciences Streptococcus Plant Pathology biology.organism_classification Maize Mycoses Animal Studies |
| Zdroj: | PLoS ONE PLoS ONE, Vol 15, Iss 10, p e0240545 (2020) |
| ISSN: | 1932-6203 |
| Popis: | The bacterial strain, EMM-1, was isolated from the rhizosphere of red maize ("Rojo Criollo") and identified as Pseudomonas protegens EMM-1 based on phylogenetic analysis of 16S rDNA, rpoB, rpoD, and gyrB gene sequences. We uncovered genes involved in the production of antimicrobial compounds like 2,4-diacetylphloroglucinol (2,4-DAPG), pyoluteorin, and lectin-like bacteriocins. These antimicrobial compounds are also produced by other fluorescent pseudomonads alike P. protegens. Double-layer agar assay showed that P. protegens EMM-1 inhibited the growth of several multidrug-resistant (MDR) bacteria, especially clinical isolates of the genera Klebsiella and β-hemolytic Streptococcus. This strain also displayed inhibitory effects against diverse fungi, such as Aspergillus, Botrytis, and Fusarium. Besides, a crude extract of inhibitory substances secreted into agar was obtained after the cold-leaching process, and physicochemical characterization was performed. The partially purified inhibitory substances produced by P. protegens EMM-1 inhibited the growth of Streptococcus sp. and Microbacterium sp., but no inhibitory effect was noted for other bacterial or fungal strains. The molecular weight determined after ultrafiltration was between 3 and 10 kDa. The inhibitory activity was thermally stable up to 60°C (but completely lost at 100°C), and the inhibitory activity remained active in a wide pH range (from 3 to 9). After treatment with a protease from Bacillus licheniformis, the inhibitory activity was decreased by 90%, suggesting the presence of proteic natural compounds. All these findings suggested that P. protegens EMM-1 is a potential source of antimicrobials to be used against pathogens for humans and plants. |
| Databáze: | OpenAIRE |
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