Vaccine-Induced Immunity in Baboons by Using DNA and Replication-Incompetent Adenovirus Type 5 Vectors Expressing a Human Immunodeficiency Virus Type 1 gag Gene
| Autor: | Jose M. Aste-Amezaga, Lynne Isopi, Ling Chen, David B. Volkin, Liming Guan, Aimin Tang, Tong-Ming Fu, Virginia Harris, John W. Shiver, Emilio A. Emini, Danilo R. Casimiro, Lingyi Huang, Robert D. Troutman, Mary-Ellen Davies, Karen Rice, Keith A. Wilson, Daniel C. Freed, Henryk Mach, Romnie Long, Krishna K. Murthy, Denise K. Nawrocki, William Hurni, Robert K. Evans |
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| Rok vydání: | 2003 |
| Předmět: |
T-Lymphocytes
viruses Immunology Dose-Response Relationship Immunologic Biology medicine.disease_cause Microbiology Virus Defective virus Adenoviridae chemistry.chemical_compound Immune system Immunity Virology Vaccines and Antiviral Agents medicine Animals Cytotoxic T cell AIDS Vaccines Immunogenicity Defective Viruses Genes gag chemistry Insect Science HIV-1 Vaccinia Papio |
| Zdroj: | Journal of Virology. 77:7663-7668 |
| ISSN: | 1098-5514 0022-538X |
| Popis: | The cellular immunogenicity of formulated plasmid DNA and replication-defective human adenovirus serotype 5 (Ad5) vaccine vectors expressing a codon-optimized human immunodeficiency virus type 1 gag gene was examined in baboons. The Ad5 vaccine was capable of inducing consistently strong, long-lived CD8 + -biased T-cell responses and in vitro cytotoxic activities. The DNA vaccine-elicited immune responses were weaker than those elicited by the Ad5 vaccine and highly variable; formulation with chemical adjuvants led to moderate increases in the levels of Gag-specific T cells. Increasing the DNA-primed responses with booster doses of either Ad5 or modified vaccinia virus Ankara vaccines suggests a difference in the relative levels of cytotoxic and helper responses. The implications of these results are discussed. |
| Databáze: | OpenAIRE |
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