Morphometric and histopathological evaluation of the effect of grape seed proanthocyanidin on alveolar bone loss in experimental diabetes and periodontitis

Autor: Fikret Gevrek, Hulya Toker, H. Balci Yuce, A. Lektemur Alpan, Mahfuz Elmastas
Přispěvatelé: [Toker, H. -- Alpan, A. Lektemur] Cumhuriyet Univ, Dept Periodontol, Fac Dent, Sivas, Turkey -- [Yuce, H. Balci] Gaziosmanpasa Univ, Dept Periodontol, Fac Dent, Tokat, Turkey -- [Gevrek, F.] Gaziosmanpasa Univ, Dept Histol & Embryol, Fac Med, Tokat, Turkey -- [Elmastas, M.] Gaziosmanpasa Univ, Fac Arts & Sci, Dept Chem, Tokat, Turkey, balci yuce, hatice -- 0000-0003-3574-9751, LEKTEMUR ALPAN, AYSAN -- 0000-0002-5939-4783
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Blood Glucose
Male
Vascular Endothelial Growth Factor A
0301 basic medicine
alveolar bone loss
medicine.medical_treatment
Alveolar Bone Loss
Osteoclasts
Antioxidants
chemistry.chemical_compound
0302 clinical medicine
periodontitis
diabetes
Osteoblast
Immunohistochemistry
Vascular endothelial growth factor
Matrix Metalloproteinase 8
medicine.anatomical_structure
Periodontics
Hypoxia-Inducible Factor 1
Injections
Intraperitoneal
medicine.drug
medicine.medical_specialty
Intraperitoneal injection
Streptozocin
Diabetes Mellitus
Experimental
03 medical and health sciences
Osteoclast
Internal medicine
Diabetes mellitus
Alveolar Process
medicine
Animals
Proanthocyanidins
Rats
Wistar
Periodontitis
Ligation
Dental alveolus
Inflammation
Osteoblasts
Grape Seed Extract
Tartrate-Resistant Acid Phosphatase
business.industry
Body Weight
030206 dentistry
Streptozotocin
medicine.disease
Rats
Disease Models
Animal
030104 developmental biology
Endocrinology
chemistry
grape seed proanthocyanidins
tartrate-resistant acid phosphatase
business
Popis: WOS: 000432018000022
PubMed ID: 29446089
Objective: Grape seed proanthocyanidine extract (GSPE) is a strong antioxidant derived from the grape seeds (Vitis vinifera, Terral J.F.) and has a polyphenolic structure with a wide range of biological activity. The aim of the present study was to evaluate the effects of GSPE on alveolar bone loss and histopathological changes in rats with diabetes mellitus and ligature-induced periodontitis. Material and Methods: Forty rats were divided into 6 study groups. Control (C, 6 rats) group, periodontitis (P, 6 rats) group, diabetes (D, 6 rats) group, diabetes and periodontitis (D+P, 6 rats) group, diabetes, periodontitis and 100 mg/kg/day GSPE (GSPE-100, 8 rats), and diabetes, periodontitis and 200 mg/kg/day GSPE (GSPE-200, 8 rats) group. Diabetes mellitus was induced by intraperitoneal injection of a single dose of streptozotocin (60 mg/kg). Periodontitis was induced via ligation method. Silk ligatures were placed at the mandibular right first molars. GSPE was administered by oral gavage. After 30 days, all rats were killed. Alveolar bone loss was measured morphometrically via a stereomicroscope. For histopathological analyses, Alizarin red staining, and matrix metalloproteinase (MMP)-8, vascular endothelial growth factor and hypoxia inducible factor (HIF)-1 alpha immunohistochemistry were performed. Tartrate-resistant acid phosphatase-positive osteoclast cells and relative total inflammatory cells were also determined. Results: The highest alveolar bone loss was observed in the D+P group (P < .05). GSP-200 group decreased alveolar bone loss (P < .05). The D+P group had the highest osteoclast counts, but the difference was not significant compared to the P, GSPE-100 and GSPE-200 groups (P > .05). The inflammation in the D+P group was also higher than the other groups (P < .05). The osteoblast numbers increased in the GSPE-100 and GSPE-200 groups compared to the P and D+P groups (P < .05). MMP-8 and HIF-1 levels were highest in the D+P group and GSPE significantly decreased these levels (P < .05). Conclusion: Within the limits of this animal study, it can be suggested that GSPE administration may decrease periodontal inflammation and alveolar bone loss via decreasing MMP-8 and HIF-1 alpha levels and increase osteoblastic activity in diabetic rats with experimental periodontitis.
Databáze: OpenAIRE
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