Up-regulation of lipocortin-1 and its mRNA in reactive astrocytes in kainate-lesioned rat cerebellum
| Autor: | Karen A. Young, Derek R. Marriott, Graham P. Wilkin, Lynette Mullens, Lesley A. Tannahill, Stafford L. Lightman |
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| Rok vydání: | 1994 |
| Předmět: |
Male
Blotting Western Immunology Immunocytochemistry Central nervous system Kainate receptor Biology Rats Sprague-Dawley Downregulation and upregulation Cerebellum Gene expression medicine Animals Immunology and Allergy Neurotoxin RNA Messenger Northern blot Cells Cultured Annexin A1 Kainic Acid Blotting Northern Immunohistochemistry Rats Up-Regulation Cell biology medicine.anatomical_structure Neurology Astrocytes Neurology (clinical) Neuroscience Astrocyte |
| Zdroj: | Journal of Neuroimmunology. 50:25-33 |
| ISSN: | 0165-5728 |
| DOI: | 10.1016/0165-5728(94)90211-9 |
| Popis: | We have used a combined molecular and immunocytochemical approach to examine the expression of lipocortin-1 (LC-1) in kainate-lesioned rat cerebellum. Using immunocytochemistry, Western and Northern blotting, we have shown upregulation of LC-1 mRNA and expression of LC-1 localised specifically to reactive astrocytes. These studies suggest that reactive astrocytes are a major synthetic compartment for the expression of LC-1. The well-reported immuno-suppressive effects of lipocortin(s), suggests that reactive astrocytes could serve to negatively modulate inflammatory reactions in the central nervous system. |
| Databáze: | OpenAIRE |
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