Evaluation of cardiac troponin I and T levels as markers of myocardial damage in doxorubicin-induced cardiomyopathy rats, and their relationship with echocardiographic and histological findings
| Autor: | Isabelle Giuliani, Jean-Marie Juan, Marie-Claire Oliva-Lauraire, Claude Granier, Anne Polge, Michel Dauzat, J.P Bertinchant, Michel Laprade, J.-E. de La Coussaye, C Marty-Double, Jean-Pierre Bali, J.Y Burdy, Pascale Fabbro-Peray |
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| Rok vydání: | 2003 |
| Předmět: |
Cathepsin H
medicine.medical_specialty Pathology Blotting Western Clinical Biochemistry Cardiomyopathy Cell Count Enzyme-Linked Immunosorbent Assay macromolecular substances Biochemistry Cathepsin B Troponin complex Internal medicine Troponin I Meningeal Neoplasms medicine Humans Cystatin C Neoplasm Metastasis Cerebrospinal Fluid Cardiotoxicity Leukemia biology Cumulative dose business.industry Biochemistry (medical) General Medicine medicine.disease Cathepsins Cystatins Immunohistochemistry Troponin Cysteine Endopeptidases Toxicity biology.protein Cardiology business |
| Zdroj: | Clinica Chimica Acta. 329:39-51 |
| ISSN: | 0009-8981 |
| DOI: | 10.1016/s0009-8981(03)00013-5 |
| Popis: | Background: Cardiac troponins I (cTnI) and T (cTnT) have been shown to be highly sensitive and specific markers of myocardial cell injury. We investigated the diagnostic value of cTnI and cTnT for the diagnosis of myocardial damage in a rat model of doxorubicin (DOX)-induced cardiomyopathy, and we examined the relationship between serial cTnI and cTnT with the development of cardiac disorders monitored by echocardiography and histological examinations in this model. Methods: Thirty-five Wistar rats were given 1.5 mg/kg DOX, i.v., weekly for up to 8 weeks for a total cumulative dose of 12 mg/kg BW. Ten rats received saline as a control group. cTnI was measured with Access® (ng/ml) and a research immunoassay (pg/ml), and compared with cTnT, CK-MB mass and CK. By using transthoracic echocardiography, anterior and posterior wall thickness, LV diameters and LV fractional shortening (FS) were measured in all rats before DOX or saline, and at weeks 6 and 9 after treatment in all surviving rats. Histology was performed in DOX-rats at 6 and 9 weeks after the last DOX dose and in all controls. Results: Eighteen of the DOX rats died prematurely of general toxicity during the 9-week period. End-diastolic (ED) and end-systolic (ES) LV diameters/BW significantly increased, whereas LV FS was decreased after 9 weeks in the DOX group (p |
| Databáze: | OpenAIRE |
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