B cells extract antigens at Arp2/3-generated actin foci interspersed with linear filaments
| Autor: | Dessislava Malinova, Pavel Tolar, Susan Cox, Sophie I. Roper, Laabiah Wasim, Michael Way |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
actin cytoskeleton Immunological Synapses Mouse Cytoskeleton organization Imaging Immunology and Inflammation 0302 clinical medicine Biology (General) Cytoskeleton B-Lymphocytes B cell biology Chemistry General Neuroscience immune synapse General Medicine Cell biology medicine.anatomical_structure Formins Medicine Research Article Human Model organisms QH301-705.5 Science Neuroscience(all) B-cell receptor Immunology Antigen-Presenting Cells Receptors Antigen B-Cell Mice Transgenic Infectious Disease macromolecular substances Biochemistry & Proteomics Actin-Related Protein 2-3 Complex General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Signalling & Oncogenes antigen Antigen Cell Line Tumor Immunology and Microbiology(all) medicine Animals Humans endocytosis Antigens Actin B cell receptor General Immunology and Microbiology Biochemistry Genetics and Molecular Biology(all) FOS: Clinical medicine Cell Biology Actin cytoskeleton Actins Mice Inbred C57BL HEK293 Cells 030104 developmental biology Antibody Formation biology.protein 030217 neurology & neurosurgery Structural Biology & Biophysics |
| Zdroj: | Roper, S I, Wasim, L, Malinova, D, Way, M, Cox, S & Tolar, P 2019, ' B cells extract antigens at arp2/3-generated actin foci interspersed with linear filaments ', eLife, vol. 8, e48093 . https://doi.org/10.7554/eLife.48093 eLife eLife, Vol 8 (2019) |
| DOI: | 10.25418/crick.11689302.v1 |
| Popis: | Antibody production depends on B cell internalization and presentation of antigens to helper T cells. To acquire antigens displayed by antigen-presenting cells, B cells form immune synapses and extract antigens by the mechanical activity of the acto-myosin cytoskeleton. While cytoskeleton organization driving the initial formation of the B cell synapse has been studied, how the cytoskeleton supports antigen extraction remains poorly understood. Here we show that after initial cell spreading, F-actin in synapses of primary mouse B cells and human B cell lines forms a highly dynamic pattern composed of actin foci interspersed with linear filaments and myosin IIa. The foci are generated by Arp2/3-mediated branched-actin polymerization and stochastically associate with antigen clusters to mediate internalization. However, antigen extraction also requires the activity of formins, which reside near the foci and produce the interspersed filaments. Thus, a cooperation of branched-actin foci supported by linear filaments underlies B cell mechanics during antigen extraction. |
| Databáze: | OpenAIRE |
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