Acrolein, an α,β-Unsaturated Carbonyl, Inhibits Both Growth and PSII Activity in the CyanobacteriumSynechocystissp. PCC 6803
| Autor: | Tomohito Mabuchi, Tatsuya Iwamoto, Ginga Shimakawa, Amane Makino, Chikahiro Miyake, Hiroshi Yamamoto, Toshio Sugimoto, Katsumi Amako, Ryota Saito |
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| Rok vydání: | 2013 |
| Předmět: |
Photosynthetic reaction centre
Photosystem II macromolecular substances Photosynthesis Photosystem I Photochemistry Applied Microbiology and Biotechnology Biochemistry Analytical Chemistry chemistry.chemical_compound Benzoquinones Acrolein Molecular Biology P700 Chemistry Organic Chemistry Synechocystis Photosystem II Protein Complex food and beverages General Medicine Carbon Dioxide Benzoquinone Oxygen Thylakoid Biotechnology |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 77:1655-1660 |
| ISSN: | 1347-6947 0916-8451 |
| Popis: | In this study, we sought to determine whether and how an α,β-unsaturated carbonyl, acrolein, can inhibit the growth of the cyanobacterium Synechocystis sp. PCC6803 (S. 6803). Treatment of S. 6803 with 200 µM acrolein for 3 d significantly and irreversibly inhibited its growth. To elucidate the inhibitory mechanism, we examined the effects of acrolein on photosynthesis. In contrast to dark conditions, the addition of acrolein to S. 6803 under conditions of illumination lowered the CO₂-dependent O₂ evolution rate (photosynthetic activity). Furthermore, treatment with acrolein lowered the activity reducing dimethyl benzoquinone in photosystem II (PSII). Acrolein also suppressed the reduction rate for the oxidized form of the reaction center chlorophyll of photosystem I (PSI), P700. These results indicate that acrolein inhibited PSII activity in thylakoid membranes. The addition of 200 µM acrolein to the illuminated S. 6803 cells gradually increased the steady-state level (Fs) of Chl fluorescence and decreased the quantum yield of PSII. These results suggested that acrolein damaged the acceptor side of PSII. On the other hand, acrolein did not inhibit respiration. From the above results, we gained insight into the metabolism of acrolein and its physiological effects in S. 6803. |
| Databáze: | OpenAIRE |
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