Rapid spread of Clostridium difficile NAP1/027/ST1 in Chile confirms the emergence of the epidemic strain in Latin America
| Autor: | J. Lagos, R. Flores, J. Fernández, Simon Lévesque, S. Ulloa, P. Araya, J. C. Hormazabal, C. Aguayo, J. Tognarelli, O. Duery, D. Ibáñez, B. Olivares, P. Pidal |
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| Rok vydání: | 2015 |
| Předmět: |
Adult
Male Adolescent Genotype Epidemiology Bacterial Toxins Short Report Biology Ribotyping Microbiology law.invention Young Adult law Pulsed-field gel electrophoresis Cluster Analysis Humans Chile Child Epidemics Epidemic strain Polymerase chain reaction Aged Aged 80 and over Molecular Epidemiology Molecular epidemiology Clostridioides difficile Strain (biology) Infant Newborn Infant Middle Aged Clostridium difficile Virology Hospitals Electrophoresis Gel Pulsed-Field Infectious Diseases Child Preschool Clostridium Infections Multilocus sequence typing Female Multilocus Sequence Typing |
| Zdroj: | Epidemiol Infect |
| ISSN: | 1469-4409 0950-2688 |
| DOI: | 10.1017/s0950268815000023 |
| Popis: | SUMMARYClostridium difficile infection has gained importance in recent years as a result of the rapid spread of epidemic strains, including hypervirulent strains. This study reports the molecular epidemiology of C. difficile obtained from hospitalized patients in Chile. Seven hundred and nineteen isolates of toxigenic C. difficile from 45 hospitals across the country were characterized through toxin profile, pulsed-field gel electrophoresis (PFGE), and sequencing of the tcdC gene. In addition, polymerase chain reaction (PCR) ribotyping and multilocus sequence typing (MLST) were performed on a subset of selected strains. PFGE typing of 719 isolates of C. difficile produced 60 PFGE patterns (subtypes). Subtype 1 was predominant (79% of isolates) and related to the hypervirulent strain (NAP1). Subtype 1 showed 73% relatedness with nine other subtypes, which had a similar tcdC deletion. Subtype 1 corresponded to ribotype 027 and ST1. This report shows the wide dissemination of the hypervirulent strain NAP1/027/ST1 in Chile. |
| Databáze: | OpenAIRE |
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