An extremely acidic copper-containing protein from chromaffin granules
| Autor: | R.M. Nalbandyan, H. Ch. Buniatian, N.A. Grigoryan |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Epinephrine
Biophysics chemistry.chemical_element Nerve Tissue Proteins Polypeptide chain Dopamine beta-Hydroxylase Biochemistry law.invention chemistry.chemical_compound law Dopamine medicine Animals Bovine adrenal Chromaffin Granules Adrenochrome Amino Acids Electron paramagnetic resonance Molecular Biology chemistry.chemical_classification Electron Spin Resonance Spectroscopy Cell Biology Copper Molecular Weight Enzyme chemistry Homogeneous Adrenal Medulla Chromaffin System Cattle Oxidation-Reduction medicine.drug |
| Zdroj: | Biochemical and biophysical research communications. 100(3) |
| ISSN: | 0006-291X |
| Popis: | A new copper-containing protein has been isolated in an electrophoretically homogeneous state from chromaffin granules of bovine adrenal medulla. The protein is extremely acidic (Ip∼3.5) and thermostable. It consists of one polypeptide chain of 10000 molecular weight and contains one atom of EPR-detectable type 2 copper. EPR spectra of the protein is very sensitive to pH changes. The oxidized protein is reduced by adrenaline, the latter being oxidized to adrenochrome while the reduced protein is oxidized by adrenochrome. The properties of the protein are drastically different from those of the copper enzyme, dopamine- β -hydroxylase, also obtained from chromaffin granules. However, there are many similarities in the properties of this protein and neurocuprein, a copper-containing protein isolated earlier from brain. |
| Databáze: | OpenAIRE |
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