Heat-modified citrus pectin induces apoptosis-like cell death and autophagy in HepG2 and A549 cancer cells
| Autor: | Carine Michiels, Thierry Arnould, Lionel Leclere, Pierre Van Cutsem, Francois Cote, Pierre Cambier, Maude Fransolet |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Programmed cell death
Hot Temperature food.ingredient Pectin lcsh:Medicine Apoptosis Biology food Cell Line Tumor Autophagy Humans Citrus Pectin lcsh:Science A549 cell Multidisciplinary Adenine Microfilament Proteins lcsh:R Ubiquitination food and beverages Hep G2 Cells Modified Citrus Pectin Caspase Inhibitors Cell biology Biochemistry Caspases Cancer cell Pectins lcsh:Q Macrolides Carrier Proteins Research Article |
| Zdroj: | PLoS ONE, Vol 10, Iss 3, p e0115831 (2015) Leclere, L, Fransolet, M, Cote, F, Cambier, P, Arnould, T, Van Cutsem, P & Michiels, C 2015, ' Heat-modified citrus pectin induces apoptosis-like cell death and autophagy in HepG2 and A549 cancer cells ', PLoS ONE, vol. 10, no. 3, e0115831 . https://doi.org/10.1371/journal.pone.0115831 PLoS ONE |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0115831 |
| Popis: | Cancer is still one of the leading causes of death worldwide, and finding new treatments remains a major challenge. Previous studies showed that modified forms of pectin, a complex polysaccharide present in the primary plant cell wall, possess anticancer properties. Nevertheless, the mechanism of action of modified pectin and the pathways involved are unclear. Here, we show that citrus pectin modified by heat treatment induced cell death in HepG2 and A549 cells. The induced cell death differs from classical apoptosis because no DNA cleavage was observed. In addition, Z-VAD-fmk, a pan-caspase inhibitor, did not influence the observed cell death in HepG2 cells but appeared to be partly protective in A549 cells, indicating that heat-modified citrus pectin might induce caspase-independent cell death. An increase in the abundance of the phosphatidylethanolamine-conjugated Light Chain 3 (LC3) protein and a decrease in p62 protein abundance were observed in both cell types when incubated in the presence of heat-modified citrus pectin. These results indicate the activation of autophagy. To our knowledge, this is the first time that autophagy has been revealed in cells incubated in the presence of a modified form of pectin. This autophagy activation appears to be protective, at least for A549 cells, because its inhibition with 3-methyladenine increased the observed modified pectin-induced cytotoxicity. This study confirms the potential of modified pectin to improve chemotherapeutic cancer treatments. |
| Databáze: | OpenAIRE |
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