Physiologic assessment of botulinum toxin effects in the rat larynx
Autor: | Katsuhide Inagi, Edward Schultz, Arthur A. Rodriquez, Charles N. Ford, Thomas R. Pasic, Nadine P. Connor, Diane M. Bless, Dennis M. Heisey |
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Rok vydání: | 1998 |
Předmět: |
Male
Cricoarytenoid Muscle Larynx Pathology medicine.medical_specialty Botulinum Toxins Laryngoscopy Drug Evaluation Preclinical Vocal Cords Electromyography Injections Intramuscular Spasmodic dysphonia Rats Sprague-Dawley Random Allocation Animals Medicine Denervation Voice Disorders Dose-Response Relationship Drug medicine.diagnostic_test Anti-Dyskinesia Agents business.industry Reproducibility of Results Botulinum toxin Electric Stimulation Rats Disease Models Animal medicine.anatomical_structure Otorhinolaryngology Laryngeal Muscle Anesthesia Laryngeal Muscles medicine.symptom business medicine.drug |
Zdroj: | The Laryngoscope. 108:1048-1054 |
ISSN: | 1531-4995 0023-852X |
Popis: | Objective: Botulinum toxin (BT) is a currently used treatment for spasmodic dysphonia (SD) and other related focal dystonias. The goal of this study is to provide a basis for using the rat larynx to objectively assess physiological and histological effects of BT. Study Design: Dosages and volumes of BT injection were varied and three physiological parameters were measured. These measures included: optical density of PAS-stained laryngeal muscle after electrical stimulation, which is an indirect measure of denervation, spontaneous laryngeal muscle activity, and laryngeal movement. Methods: A new microlaryngoscopic technique was developed, which made it possible to observe and manipulate the rat larynx endoscopically. Laryngeal movement and electromyographic (EMG) measures were made prior to injection and 3 days following BT injections of various dosages and volumes. Optical density measures were made 3 days after injection. Results: Significant reductions in vocal fold motion and spontaneous laryngeal muscle activity as a function of increased BT dosage were observed. In addition, the optical density of PAS-stained laryngeal muscle after electrical stimulation was increased following BT injection. Significant volume effects in optical density were observed in the lateral thyroarytenoid and lateral cricoarytenoid muscles on the contralateral side. Conclusions: The rat laryngeal model is suitable for assessing BT effects. In addition, the three physiological variables provided useful and reliable measures of laryngeal function. It is the authors' intention to use the rat laryngeal model to further examine the physiological and histological effects of BT with the goal of developing new methods for the treatment of patients with SD and other focal dystonias. |
Databáze: | OpenAIRE |
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