Comprehensive Analysis of Tissue Preservation and Recording Quality from Chronic Multielectrode Implants
| Autor: | Koichi Sameshima, Marco Aurelio M. Freire, José R. Santos, Joanilson S. Guimarães, Sidarta Ribeiro, Antonio Pereira, Nelson A. M. Lemos, Edgard Morya, Jean Faber, Miguel A. L. Nicolelis |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Male
Pathology medicine.medical_specialty lcsh:Medicine Neurophysiology Inflammation Biology 03 medical and health sciences 0302 clinical medicine Model Organisms medicine Animals Rats Wistar lcsh:Science Genes Immediate-Early Cytoskeleton 030304 developmental biology 0303 health sciences Multidisciplinary Tissue Preservation Neuronal Morphology lcsh:R Motor Cortex Histology Animal Models Rats Electrophysiology Functional integrity Neuroanatomy medicine.anatomical_structure Cerebral cortex Cellular Neuroscience Immunohistochemistry Rat lcsh:Q medicine.symptom 030217 neurology & neurosurgery Motor cortex Research Article Neuroscience |
| Zdroj: | PLoS ONE PLoS ONE, Vol 6, Iss 11, p e27554 (2011) |
| ISSN: | 1932-6203 |
| Popis: | Multielectrodes have been used with great success to simultaneously record the activity of neuronal populations in awake, behaving animals. In particular, there is great promise in the use of this technique to allow the control of neuroprosthetic devices by human patients. However, it is crucial to fully characterize the tissue response to the chronic implants in animal models ahead of the initiation of human clinical trials. Here we evaluated the effects of unilateral multielectrode implants on the motor cortex of rats weekly recorded for 1-6 months using several histological methods to assess metabolic markers, inflammatory response, immediate-early gene (IEG) expression, cytoskeletal integrity and apoptotic profiles. We also investigated the correlations between each of these features and firing rates, to estimate the impact of post-implant time on neuronal recordings. Overall, limited neuronal loss and glial activation were observed on the implanted sites. Reactivity to enzymatic metabolic markers and IEG expression were not significantly different between implanted and non-implanted hemispheres. Multielectrode recordings remained viable for up to 6 months after implantation, and firing rates correlated well to the histochemical and immunohistochemical markers. Altogether, our results indicate that chronic tungsten multielectrode implants do not substantially alter the histological and functional integrity of target sites in the cerebral cortex. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|