Transfer of specific immunity from donor to recipient of an allogeneic bone marrow graft: evidence for donor origin of the antibody producing cells
| Autor: | F. E. Zwaan, J. Labadie, N. H. Dijkstra, J. M. J. J. Vossen, M.J.D. van Tol, M.E. van der Kaaden, G. G. de Lange, C. M. Jol van der Zijde |
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| Rok vydání: | 1992 |
| Předmět: |
Adult
Male Adoptive cell transfer Immunoblotting Enzyme-Linked Immunosorbent Assay chemical and pharmacologic phenomena Subclass Random Allocation Immunoglobulin Gm Allotypes Tetanus Toxoid Animals Humans Medicine Prospective Studies Child Bone Marrow Transplantation biology business.industry Isoelectric focusing Helix Snails Hematology medicine.disease Antibodies Bacterial Tissue Donors Leukemia Phenotype surgical procedures operative medicine.anatomical_structure Immunization Immunoglobulin G Antibody Formation Hemocyanins Immunology Humoral immunity biology.protein Female Bone marrow Isoelectric Focusing Antibody business |
| Zdroj: | British Journal of Haematology. 82:437-444 |
| ISSN: | 1365-2141 0007-1048 |
| Popis: | A rapid recovery of specific humoral immunity in the recipient of an allogeneic bone marrow transplantation (BMT) can be observed after immunization of the donor before graft sampling. This has been attributed to transfer of specific immunity from donor to recipient. However, to maintain the concept of transfer the origin of the antibody producing cells in the recipient after BMT must be demonstrated. To this end, donor-recipient pairs with differences in Gm-allotypes were selected and immunized before BMT with the neo-antigen Helix pomatia haemocyanin (HPH) according to three immunization protocols. Additionally, the recipients were immunized at day 42 after BMT. Serum samples were weekly obtained from the recipients in the first 100 d after BMT. The origin of HPH-specific antibody producing cells was assessed by two approaches: (1) determination of the Gm-allotypes of anti-HPH antibodies within a distinct IgG subclass, (2) analysis of anti-HPH antibody spectrotypes by isoelectric focusing combined with immunoblotting. The results obtained with these two approaches show concordance in most instances and led to the conclusion that the antibody producing cells are of donor origin. |
| Databáze: | OpenAIRE |
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