Primary cilia are sensors of electrical field stimulation to induce osteogenesis of human adipose-derived stem cells
| Autor: | Shaobo Cai, Alison Amos, Josephine C. Bodle, Susan H. Bernacki, Mehdi S. Hamouda, Greg McCarty, Elizabeth G. Loboa, Pattie S. Mathieu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Cell Survival chemistry.chemical_element Adipose tissue Calcium Biochemistry 03 medical and health sciences 0302 clinical medicine RNA interference Osteogenesis Internal medicine Organelle Genetics medicine Humans Cilia RNA Small Interfering Molecular Biology Cells Cultured Regulation of gene expression Cilium Research Stem Cells Electric Stimulation Cell biology 030104 developmental biology Endocrinology chemistry Adipose Tissue Gene Expression Regulation RNA Interference Stem cell Signal transduction 030217 neurology & neurosurgery Biomarkers Biotechnology |
| Popis: | In this study, we report for the first time that the primary cilium acts as a crucial sensor for electrical field stimulation (EFS)-enhanced osteogenic response in osteoprogenitor cells. In addition, primary cilia seem to functionally modulate effects of EFS-induced cellular calcium oscillations. Primary cilia are organelles that have recently been implicated to play a crucial sensor role for many mechanical and chemical stimuli on stem cells. Here, we investigate the role of primary cilia in EFS-enhanced osteogenic response of human adipose-derived stem cells (hASCs) by knocking down 2 primary cilia structural proteins, polycystin-1 and intraflagellar protein-88. Our results indicate that structurally integrated primary cilia are required for detection of electrical field signals in hASCs. Furthermore, by measuring changes of cytoplasmic calcium concentration in hASCs during EFS, our findings also suggest that primary cilia may potentially function as a crucial calcium-signaling nexus in hASCs during EFS.-Cai, S., Bodle, J. C., Mathieu, P. S., Amos, A., Hamouda, M., Bernacki, S., McCarty, G., Loboa, E. G. Primary cilia are sensors of electrical field stimulation to induce osteogenesis of human adipose-derived stem cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|