Intracranial self-stimulation-reward or immobilization-aversion had different effects on neurite extension and the ERK pathway in neurotransmitter-sensitive mutant PC12 cells
| Autor: | Shigeki Inoue, Yutaka Gomita, Toshiaki Sendo, Hiroaki Araki, Satoru Esumi, Yoshihisa Kitamura, Yoshio Kano, Hirotoshi Motoda, Soichiro Ushio |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Male
Pleasure MAPK/ERK pathway Neurite MAP Kinase Signaling System Neuronal signal transduction Neurogenesis Emotions Stimulation PC12 Cells 03 medical and health sciences Behavioral Neuroscience chemistry.chemical_compound 0302 clinical medicine Reward Nerve Growth Factor Nitriles Butadienes Neurites medicine Extracellular Animals Enzyme Inhibitors Rats Wistar Extracellular Signal-Regulated MAP Kinases Neurotransmitter 030304 developmental biology 0303 health sciences Behavior Animal Antagonist Rats chemistry Neuroscience 030217 neurology & neurosurgery Acetylcholine medicine.drug |
| Zdroj: | Behavioural Brain Research. 396:112920 |
| ISSN: | 0166-4328 |
| Popis: | Various actions trigger pleasure (reward) or aversion (punishment) as emotional responses. Emotional factors that negatively affect brain neural control processes for long periods of time might cause various mental diseases by inducing neuronal changes. In the present study, newly developed PC12m12 cells which are highly sensitivity to neurotransmitters such as acetylcholine (ACh), were used. Exposing the cells to plasma from rats that had been subjected to intracranial self-stimulation (ICSS) markedly upregulated neurite outgrowth. In addition, voluntary running in a wheel or forced on a rotating rod was used to induce behavioral excitation in rats, and examinations of their plasma confirmed that the ICSS-induced neurite outgrowth was not associated with the ICSS behavior itself. Furthermore, immunoblotting and treatment with U0126, an ERK (extracellular signal-regulated kinase) antagonist, showed that the ICSS-induced neurite outgrowth was related to neuronal ERK activity. Exposing the same cells to plasma from rats that had been subjected to immobilization (IMM) also increased neurite outgrowth. Although the degree of enhancement was not as great as that seen after the ICSS rat plasma treatment, it was less than that observed after treatment with ACh as a positive control. These results indicate that ICSS or IMM lead to varying degrees of morphological changes, such as enhanced neurite outgrowth, in PC12m12 cells, but the neuronal signal transduction pathways underlying these effects differ; i.e.,the former morphological change might involve the activation of the ERK pathway, whereas the latter changes might not. Using PC12m12 cells which exhibit sensitivity to neurotransmitters, it might be possible to clarify the pathogeneses of mental diseases at the neuronal level and search for therapeutic drugs. |
| Databáze: | OpenAIRE |
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