Silica-based 2-(N,N-dimethylamino)-1,3-propanediol hydrophilic interaction liquid chromatography stationary phase for separating cephalosporins and carbapenems
| Autor: | Lingping Cheng, Xiaohui Cai, Changhu Chu, Ruiqiang Guo, Renhua Liu, John A. Palasota, Wei Yin, Huihui Chai |
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| Rok vydání: | 2015 |
| Předmět: |
Azides
Cycloaddition Reaction Chemical polarity Hydrophilic interaction chromatography chemistry.chemical_element Silicon Dioxide Biochemistry Combinatorial chemistry Copper Cycloaddition Cephalosporins Analytical Chemistry Carbapenems chemistry Propylene Glycols Covalent bond Alkynes polycyclic compounds medicine Moiety Organic chemistry Theoretical plate Inosine Chromatography High Pressure Liquid medicine.drug |
| Zdroj: | Analytical and Bioanalytical Chemistry. 407:6217-6220 |
| ISSN: | 1618-2650 1618-2642 |
| DOI: | 10.1007/s00216-015-8779-y |
| Popis: | A silica-based stationary phase bearing both hydrophilic hydroxyl and amino groups was developed by covalently bonding a small molecular N,N-dimethylamino 1,3-propanediol moiety onto silica beads via copper(I)-catalyzed Huisgen azide-alkyne 1,3-dipolar cycloaddition (CuAAC). This new stationary phase showed good HILIC characteristics and high column efficiency (the theoretical plate number is up to 37000 plates m(-1) in the case of inosine) in the separation of polar compounds, such as nucleosides and bases, organic acids, cephalosporins, and carbapenems. |
| Databáze: | OpenAIRE |
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