CRISPR-TAPE: protein-centric CRISPR guide design for targeted proteome engineering
| Autor: | Henry J Benns, Edward W. Tate, Daniel Paolo Anderson, Matthew A. Child |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
chemistry.chemical_classification
0303 health sciences Computer science food and beverages Computational biology 010402 general chemistry 01 natural sciences 0104 chemical sciences Amino acid Molecular engineering 03 medical and health sciences ComputingMethodologies_PATTERNRECOGNITION chemistry Proteome CRISPR Guide RNA 030304 developmental biology |
| Popis: | Rational molecular engineering of proteins with CRISPR-based approaches is challenged by the gene-centric nature of gRNA design tools. To address this, we have developed CRISPR-TAPE, a protein-centric gRNA design algorithm that allows users to target specific residues, or amino acid types within proteins. gRNA outputs can be customized to support maximal efficacy of homology-directed repair for engineering purposes, removing time consuming post-hoc curation, simplifying gRNA outputs, and reducing CPU times. |
| Databáze: | OpenAIRE |
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